Supplementary Materials

Supplementary Materials for:

Deletions in the cytoplasmic domain of iRhom1 and iRhom2 promote shedding of the TNF receptor by the protease ADAM17

Sathish K. Maney, David R. McIlwain, Robin Polz, Aleksandra A. Pandyra, Balamurugan Sundaram, Dorit Wolff, Kazuhito Ohishi, Thorsten Maretzky, Matthew A. Brooke, Astrid Evers, Ananda A. Jaguva Vasudevan, Nima Aghaeepour, Jürgen Scheller, Carsten Münk, Dieter Häussinger, Tak W. Mak, Garry P. Nolan, David P. Kelsell, Carl P. Blobel, Karl S. Lang, Philipp A. Lang*

*Corresponding author. E-mail: philipp.lang{at}med.uni-duesseldorf.de

This PDF file includes:

  • Fig. S1. Cloning of CPR screen–identified iRhom versions.
  • Fig. S2. TNFR1 and TNFR2 abundance in iRhom-expressing cells.
  • Fig. S3. ADAM17 mediates cleavage of TNFRs.
  • Fig. S4. Stable knockdown of ADAM17 prevents ΔN-iRhom–dependent TNFR shedding.
  • Fig. S5. Localization patterns of truncated iRhoms and association with ADAM17.
  • Fig. S6. Increased ADAM17 activity associated with N-terminal truncated iRhom2.

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Citation: S. K. Maney, D. R. McIlwain, R. Polz, A. A. Pandyra, B. Sundaram, D. Wolff, K. Ohishi, T. Maretzky, M. A. Brooke, A. Evers, A. A. J. Vasudevan, N. Aghaeepour, J. Scheller, C. Münk, D. Häussinger, T. W. Mak, G. P. Nolan, D. P. Kelsell, C. P. Blobel, K. S. Lang, P. A. Lang, Deletions in the cytoplasmic domain of iRhom1 and iRhom2 promote shedding of the TNF receptor by the protease ADAM17. Sci. Signal. 8, ra109 (2015).

© 2015 American Association for the Advancement of Science