Supplementary Materials for:

The mode of agonist binding to a G protein–coupled receptor switches the effect that voltage changes have on signaling

Andreas Rinne, Juan Carlos Mobarec, Martyn Mahaut-Smith, Peter Kolb, Moritz Bünemann*

*Corresponding author. E-mail: moritz.buenemann{at}staff.uni-marburg.de

This PDF file includes:

• Fig. S1. Analysis of the voltage sensitivity of the M₁ receptor when uncoupled from G_q proteins.
• Fig. S2. Analysis of the voltage sensitivity of the M₅ receptor.
• Fig. S3. Variations of the calculated ligand binding poses.
• Fig. S4. Analysis of the voltage sensitivity of the N6.52A and D3.32E mutant M₃ receptors.
• Fig. S5. Analysis of the voltage sensitivity of the choline-activated M₃ receptor.
• Fig. S6. Analysis of the voltage sensitivity of the N6.52Q mutant M₁ receptor.
• Fig. S7. The N6.52Q mutation in the M₃ receptor reduces its affinity for ligands.
• Table S1. Average distances between residues 6.52 of TM6 or 3.32 of TM3 and ligands in the wild-type and mutant M₃ receptors.

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Citation: A. Rinne, J. C. Mobarec, M. Mahaut-Smith, P. Kolb, M. Bünemann, The mode of agonist binding to a G protein–coupled receptor switches the effect that voltage changes have on signaling. Sci. Signal. 8, ra110 (2015).

© 2015 American Association for the Advancement of Science