Supplementary Materials

Supplementary Materials for:

The mode of agonist binding to a G protein–coupled receptor switches the effect that voltage changes have on signaling

Andreas Rinne, Juan Carlos Mobarec, Martyn Mahaut-Smith, Peter Kolb, Moritz Bünemann*

*Corresponding author. E-mail: moritz.buenemann{at}staff.uni-marburg.de

This PDF file includes:

  • Fig. S1. Analysis of the voltage sensitivity of the M1 receptor when uncoupled from Gq proteins.
  • Fig. S2. Analysis of the voltage sensitivity of the M5 receptor.
  • Fig. S3. Variations of the calculated ligand binding poses.
  • Fig. S4. Analysis of the voltage sensitivity of the N6.52A and D3.32E mutant M3 receptors.
  • Fig. S5. Analysis of the voltage sensitivity of the choline-activated M3 receptor.
  • Fig. S6. Analysis of the voltage sensitivity of the N6.52Q mutant M1 receptor.
  • Fig. S7. The N6.52Q mutation in the M3 receptor reduces its affinity for ligands.
  • Table S1. Average distances between residues 6.52 of TM6 or 3.32 of TM3 and ligands in the wild-type and mutant M3 receptors.

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Citation: A. Rinne, J. C. Mobarec, M. Mahaut-Smith, P. Kolb, M. Bünemann, The mode of agonist binding to a G protein–coupled receptor switches the effect that voltage changes have on signaling. Sci. Signal. 8, ra110 (2015).

© 2015 American Association for the Advancement of Science