Supplementary Materials

Supplementary Materials for:

Chaperoning epigenetics: FKBP51 decreases the activity of DNMT1 and mediates epigenetic effects of the antidepressant paroxetine

Nils C. Gassen,* Gabriel R. Fries, Anthony S. Zannas, Jakob Hartmann, Jürgen Zschocke, Kathrin Hafner, Tania Carrillo-Roa, Jessica Steinbacher, S. Nicole Preißinger, Lianne Hoeijmakers, Matthias Knop, Frank Weber, Stefan Kloiber, Susanne Lucae, George P. Chrousos, Thomas Carell, Marcus Ising, Elisabeth B. Binder, Mathias V. Schmidt, Joëlle Rüegg, Theo Rein*

*Corresponding author. E-mail: ncgassen{at} (N.C.G.); theorein{at} (T.R.)

This PDF file includes:

  • Fig. S1. DNMT protein interaction analysis.
  • Fig. S2. FKBP51 and FKBP52 differentially affect the phosphorylation and activity of DNMT1.
  • Fig. S3. Paroxetine promotes FKBP51 binding to CDK5.
  • Fig. S4. FKBP51 shapes the effect of paroxetine on DNMT1.
  • Fig. S5. The effect of amitriptyline and haloperidol on the phosphorylation of DNMT1.
  • Fig. S6. The effect of amitriptyline on Bdnf promoter IV methylation.
  • Fig. S7. DNA methylation of Bdnf inversely correlates with the abundance of FKBP5 mRNA.
  • Fig. S8. Cellular and clinical change in the abundance of phosphorylated DNMT1 in PBMCs in response to antidepressants.
  • Table S1. Pyrosequencing results of Bdnf promoter methylation analyses in wild-type and 51KO mice.
  • Table S2. Statistical details of ANOVA analysis by figure panel.
  • Table S3. Statistical details of Student’s t test or Mann-Whitney analysis by figure panel.
  • Table S4. Primers for bisulfite PCR and pyrosequencing.
  • Data file S1. Blot collections for Fig. 1 and figs. S1 and S3.
  • Data file S2. Blot collections for Fig. 2.
  • Data file S3. Blot collections for Fig. 3.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.83 MB

Citation: N. C. Gassen, G. R. Fries, A. S. Zannas, J. Hartmann, J. Zschocke, K. Hafner, T. Carrillo-Roa, J. Steinbacher, S. N. Preißinger, L. Hoeijmakers, M. Knop, F. Weber, S. Kloiber, S. Lucae, G. P. Chrousos, T. Carell, M. Ising, E. B. Binder, M. V. Schmidt, J. Rüegg, T. Rein, Chaperoning epigenetics: FKBP51 decreases the activity of DNMT1 andmediates epigenetic effects of the antidepressant paroxetine. Sci. Signal. 8, ra119 (2015).

© 2015 American Association for the Advancement of Science