Supplementary Materials

Supplementary Materials for:

Inhibition of the kinase ITK in a mouse model of asthma reduces cell death and fails to inhibit the inflammatory response

Yonglian Sun, Ivan Peng, Joshua D. Webster, Eric Suto, Justin Lesch, Xiumin Wu, Kate Senger, George Francis, Kathy Barrett, Jenna L. Collier, Jason D. Burch, Meijuan Zhou, Yuan Chen, Connie Chan, Jeff Eastham-Anderson, Hai Ngu, Olga Li, Tracy Staton, Charles Havnar, Allan Jaochico, Janet Jackman, Surinder Jeet, Lorena Riol-Blanco, Lawren C. Wu, David F. Choy, Joseph R. Arron, Brent S. McKenzie, Nico Ghilardi, Moulay Hicham Alaoui Ismaili, Zhonghua Pei, Jason DeVoss, Cary D. Austin, Wyne P. Lee, Ali A. Zarrin*

*Corresponding author. E-mail: zarrin.ali{at}gene.com

This PDF file includes:

  • Fig. S1. Itk−/− mice are resistant to OVA-induced airway inflammation.
  • Fig. S2. The ITK inhibitor GNE-7056 fails to show efficacy in established OVA-induced airway inflammation.
  • Fig. S3. Treatment with GNE-7056 during priming inhibits the TH2-type response.
  • Fig. S4. Inhibition of ITK does not affect the migration of T cells to the lung.
  • Fig. S5. The ITK inhibitor GNE-2125 promotes TH2-type responses and inhibits apoptosis.
  • Fig. S6. The ITK inhibitor GNE-7056 does not induce lymphoid hyperplasia in naïve mice.
  • Fig. S7. ITK inhibitors do not affect the AICD of CD4+ T cells in the presence of anti-FasL antibody.
  • Fig. S8. The suppression of AICD in T cells by the ITK inhibitor is not rescued by IL-2.
  • Fig. S9. GNE-7056 does not change the numbers of Treg cells in the spleen or DLNs.
  • Table S1. Effects of ITK inhibitors on kinases in immune cells.

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Citation: Y. Sun, I. Peng, J. D. Webster, E. Suto, J. Lesch, X. Wu, K. Senger, G. Francis, K. Barrett, J. L. Collier, J. D. Burch, M. Zhou, Y. Chen, C. Chan, J. Eastham-Anderson, H. Ngu, O. Li, T. Staton, C. Havnar, A. Jaochico, J. Jackman, S. Jeet, L. Riol-Blanco, L. C. Wu, D. F. Choy, J. R. Arron, B. S. McKenzie, N. Ghilardi, M. H. A. Ismaili, Z. Pei, J. DeVoss, C. D. Austin, W. P. Lee, A. A. Zarrin, Inhibition of the kinase ITK in a mouse model of asthma reduces cell death and fails to inhibit the inflammatory response. Sci. Signal. 8, ra122 (2015).

© 2015 American Association for the Advancement of Science