Supplementary Materials
Supplementary Materials for:
Distinct profiles of functional discrimination among G proteins determine the actions of G protein–coupled receptors
Ikuo Masuho, Olga Ostrovskaya, Grant M. Kramer, Christopher D. Jones, Keqiang Xie, Kirill A. Martemyanov
*Corresponding author. E-mail: kirill{at}scripps.edu
This PDF file includes:
- Fig. S1. Characterization of the performance of the Nluc-based BRET assay.
- Fig. S2. Effects of Ric-8A and Ric-8B on the expression of Gα subunits and their responsiveness to agonist.
- Fig. S3. Optimization of the stoichiometry of Gα and Venus-Gβγ.
- Fig. S4. Traces showing real-time activity measurements of 14 different G proteins.
- Fig. S5. The plasma membrane localization of Venus-Gβγ is dependent on coexpression with Gα subunits to ensure 1:1 complex stoichiometry.
- Fig. S6. Exogenous GPCR and Gα stimulate BRET responses in the assay.
- Fig. S7. The abundances of heterotrimers of Gα and Venus-Gβγ in cells transiently transfected with plasmids encoding 14 different Gα subunits are similar.
- Fig. S8. GPCR responses are within the dynamic range of the assay.
- Fig. S9. Fingerprinting of the deactivation phase.
- Fig. S10. Direct comparison of the agonist-induced coupling of M1R to different G proteins.
- Fig. S11. Characterization of DOR fingerprints.
Technical Details
Format: Adobe Acrobat PDF
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Citation: I. Masuho, O. Ostrovskaya, G. M. Kramer, C. D. Jones, K. Xie, K. A. Martemyanov, Distinct profiles of functional discrimination among G proteins determine the actions of G protein–coupled receptors. Sci. Signal. 8, ra123 (2015).
