Supplementary Materials

Supplementary Materials for:

Distinct profiles of functional discrimination among G proteins determine the actions of G protein–coupled receptors

Ikuo Masuho, Olga Ostrovskaya, Grant M. Kramer, Christopher D. Jones, Keqiang Xie, Kirill A. Martemyanov

*Corresponding author. E-mail: kirill{at}scripps.edu

This PDF file includes:

  • Fig. S1. Characterization of the performance of the Nluc-based BRET assay.
  • Fig. S2. Effects of Ric-8A and Ric-8B on the expression of Gα subunits and their responsiveness to agonist.
  • Fig. S3. Optimization of the stoichiometry of Gα and Venus-Gβγ.
  • Fig. S4. Traces showing real-time activity measurements of 14 different G proteins.
  • Fig. S5. The plasma membrane localization of Venus-Gβγ is dependent on coexpression with Gα subunits to ensure 1:1 complex stoichiometry.
  • Fig. S6. Exogenous GPCR and Gα stimulate BRET responses in the assay.
  • Fig. S7. The abundances of heterotrimers of Gα and Venus-Gβγ in cells transiently transfected with plasmids encoding 14 different Gα subunits are similar.
  • Fig. S8. GPCR responses are within the dynamic range of the assay.
  • Fig. S9. Fingerprinting of the deactivation phase.
  • Fig. S10. Direct comparison of the agonist-induced coupling of M1R to different G proteins.
  • Fig. S11. Characterization of DOR fingerprints.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.27 MB


Citation: I. Masuho, O. Ostrovskaya, G. M. Kramer, C. D. Jones, K. Xie, K. A. Martemyanov, Distinct profiles of functional discrimination among G proteins determine the actions of G protein–coupled receptors. Sci. Signal. 8, ra123 (2015).

© 2015 American Association for the Advancement of Science