Supplementary Materials

Supplementary Materials for:

Loss of FTO in adipose tissue decreases Angptl4 translation and alters triglyceride metabolism

Chao-Yung Wang,* Shian-Sen Shie, Ming-Shien Wen, Kuo-Chun Hung, I-Chang Hsieh, Ta-Sen Yeh, Delon Wu

*Corresponding author. E-mail: cwang{at}

This PDF file includes:

  • Fig. S1. FTO protein distribution in human and mice.
  • Fig. S2. FTO abundance after 2 and 14 days on a high-fat diet.
  • Fig. S3. Generation of adipocyte-specific FTO knockout mice.
  • Fig. S4. Analysis of tibial length, adipocyte size, energy expenditure, thyroid function, body weight, and paired feeding experiments.
  • Fig. S5. Relative mRNA expression of brown fat genes in AFO and wild-type mice.
  • Fig. S6. Glucose tolerance and insulin sensitivity.
  • Fig. S7. Lipidomics analysis of white fat tissue in AFO and wild-type mice.
  • Fig. S8. FTO protein abundance during adipocyte differentiation.
  • Fig. S9. LPL-mediated lipoprotein-dependent fatty acid uptake.
  • Fig. S10. RNA immunoprecipitation experiments.
  • Fig. S11. RNA expression in white adipose tissue.
  • Fig. S12. RNA EMSAs for Angptl4 mRNA and FTO protein.
  • Fig. S13. DAA treatment and Angptl4 abundance in AFO adipocytes.
  • Fig. S14. m6A pattern in Angptl4 mRNA.
  • Fig. S15. Polysome profiles and Angptl4 RNA abundance in control and FTO knockdown MCF-7 cell lines.
  • Fig. S16. Serum triglyceride concentrations in wild-type and AFO mice after inguinal fat pad injections of control adenovirus or adenovirus expressing Angptl4.

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Citation: C.-Y. Wang, S.-S. Shie, M.-S. Wen, K.-C. Hung, I.-C. Hsieh, T.-S. Yeh, D. Wu, Loss of FTO in adipose tissue decreases Angptl4 translation and alters triglyceride metabolism. Sci. Signal. 8, ra127 (2015).

© 2015 American Association for the Advancement of Science