Supplementary Materials

Supplementary Materials for:

Depletion of H2S during obesity enhances store-operated Ca2+ entry in adipose tissue macrophages to increase cytokine production

Gopal V. Velmurugan, Huiya Huang, Hongbin Sun, Joseph Candela, Mukesh K. Jaiswal, Kenneth D. Beaman, Megumi Yamashita, Murali Prakriya, Carl White*

*Corresponding author. E-mail: carl.white{at}rosalindfranklin.edu

This PDF file includes:

  • Fig. S1. LPS increases the abundance of CSE but not CBS.
  • Fig. S2. LPS has no effect on the abundances of STIM and Orai isoforms.
  • Fig. S3. Inhibition of endogenous H2S production increases SOCE.
  • Fig. S4. Analysis of the knockdown of STIM1 in RAW264.7 cells.
  • Fig. S5. SOCE is enhanced in ATMs from obese mice.
  • Fig. S6. Western blotting analysis of STIM1-YFP and Orai-CFP proteins in transfected HEK 293 cells.
  • Fig. S7. Analysis of the knockdown of Orai3 in RAW264.7 cells.

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Citation: G. V. Velmurugan, H. Huang, H. Sun, J. Candela, M. K. Jaiswal, K. D. Beaman, M. Yamashita, M. Prakriya, C. White, Depletion of H2S during obesity enhances store-operated Ca2+ entry in adipose tissue macrophages to increase cytokine production. Sci. Signal. 8, ra128 (2015).

© 2015 American Association for the Advancement of Science