Supplementary Materials

Supplementary Materials for:

A calcium-accumulating region, CAR, in the channel Orai1 enhances Ca2+ permeation and SOCE-induced gene transcription

Irene Frischauf, Vasilina Zayats, Michael Deix, Anna Hochreiter, Isaac Jardin, Martin Muik, Barbara Lackner, Barbora Svobodová, Teresa Pammer, Monika Litviňuková, Amrutha Arumbakam Sridhar, Isabella Derler, Ivan Bogeski, Christoph Romanin, Rüdiger H. Ettrich,* Rainer Schindl*

*Corresponding author. E-mail: rainer.schindl{at}jku.at (R.S.); ettrich{at}nh.cas.cz (R.H.E.)

This PDF file includes:

  • Fig. S1. Modeling human Orai1 with molecular dynamics simulations.
  • Fig. S2. Altered cytosolic Ca2+ concentration in cells expressing STIM1 and the Orai1 CAR mutant.
  • Fig. S3. Loop1 structure of Orai1 channels.
  • Fig. S4. Cross-linking assays and molecular dynamics simulations with mutants of loop1 and loop3 of Orai1.
  • Fig. S5. Orai1-mediated SOCE currents in mutants with loop3 cross-linking.
  • Fig. S6. Intramolecular coupling of loop1 and loop3 by electrostatic interactions between Asp114 and Lys214.
  • Legend for movie S1

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.17 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 and centering script. Molecular dynamics simulation of dynamic Ca2+ binding to CAR.

[Download Movie S1 and Script]


Citation: I. Frischauf, V. Zayats, M. Deix, A. Hochreiter, I. Jardin, M. Muik, B. Lackner, B. Svobodová, T. Pammer, M. Litviňuková, A. A. Sridhar, I. Derler, I. Bogeski, C. Romanin, R. H. Ettrich, R. Schindl, A calcium-accumulating region, CAR, in the channel Orai1 enhances Ca2+ permeation and SOCE-induced gene transcription. Sci. Signal. 8, ra131 (2015).

© 2015 American Association for the Advancement of Science