Supplementary Materials

Supplementary Materials for:

Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia

José L. Moreno, Patricia Miranda-Azpiazu, Aintzane García-Bea, Jason Younkin, Meng Cui, Alexey Kozlenkov, Ariel Ben-Ezra, Georgios Voloudakis, Amanda K. Fakira, Lia Baki, Yongchao Ge, Anastasios Georgakopoulos, José A. Morón, Graeme Milligan, Juan F. López-Giménez, Nikolaos K. Robakis, Diomedes E. Logothetis, J. Javier Meana,* Javier González-Maeso*

*Corresponding author. E-mail: javier.meana{at}ehu.eus (J.J.M.); jgmaeso{at}vcu.edu (J.G.-M.)

This PDF file includes:

  • Fig. S1. Effect of ATP on Ca2+ release.
  • Fig. S2. Relative abundances of eYFP- and mCherry-tagged constructs in HEK 293 cells.
  • Fig. S3. Concentration-response curves of LY404039 and ʟ-glutamate in HEK 293 cells.
  • Fig. S4. LY379268 has no effect on Ca2+ release in cells coexpressing 5-HT2C and mGlu2 receptors.
  • Fig. S5. Characterization of 5-HT2A, 5-HT2C, mGlu2, and mGlu3 constructs.
  • Fig. S6. Measurement of molecular interactions between eYFP- and mCherry-tagged constructs by FCM-based FRET assay.
  • Fig. S7. Radioligand binding assays.
  • Fig. S8. Characterization of 3-FRET constructs.
  • Fig. S9. Western blotting in frontal cortex of wild-type and 5-HT2A knockout mice.
  • Table S1. Demographic characteristics of schizophrenic subjects and controls.
  • Table S2. [35S]GTPγS binding in postmortem human brain samples.

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Citation: J. L. Moreno, P. Miranda-Azpiazu, A. García-Bea, J. Younkin, M. Cui, A. Kozlenkov, A. Ben-Ezra, G. Voloudakis, A. K. Fakira, L. Baki, Y. Ge, A. Georgakopoulos, J. A. Morón, G. Milligan, J. F. López-Giménez, N. K. Robakis, D. E. Logothetis, J. J. Meana, J. González-Maeso, Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia. Sci. Signal. 9, ra5 (2016).

© 2016 American Association for the Advancement of Science