Supplementary Materials

Supplementary Materials for:

Cholesterol modulates Orai1 channel function

Isabella Derler,* Isaac Jardin, Peter B. Stathopulos, Martin Muik, Marc Fahrner, Vasilina Zayats, Saurabh K. Pandey, Michael Poteser, Barbara Lackner, Marketa Absolonova, Rainer Schindl, Klaus Groschner, Rüdiger Ettrich, Mitsu Ikura, Christoph Romanin*

*Corresponding author. E-mail: isabella.derler{at} (I.D.); christoph.romanin{at} (C.R.)

This PDF file includes:

  • Fig. S1. Sequence alignment of the ETON region and TM1 in Orai1, Orai2, Orai3, and dOrai.
  • Fig. S2. The colocalization of STIM1 233–474 and FRET of STIM1 with Orai1 L74I are similar to wild-type Orai1.
  • Fig. S3. Orai1 L74I and Y80S are not affected by filipin.
  • Fig. S4. CD spectra display reduced α-helicity of the Orai1 N-terminal peptide.

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Citation: I. Derler, I. Jardin, P. B. Stathopulos, M. Muik, M. Fahrner, V. Zayats, S. K. Pandey, M. Poteser, B. Lackner, M. Absolonova, R. Schindl, K. Groschner, R. Ettrich, M. Ikura, C. Romanin, Cholesterol modulates Orai1 channel function. Sci. Signal. 9, ra10 (2016).

© 2016 American Association for the Advancement of Science