Supplementary Materials

Supplementary Materials for:

Identification of Fic-1 as an enzyme that inhibits bacterial DNA replication by AMPylating GyrB, promoting filament formation

Canhua Lu, Ernesto S. Nakayasu, Li-Qun Zhang,* Zhao-Qing Luo*

*Corresponding author. E-mail: zhanglq{at}cau.edu.cn (L.-Q.Z.); luoz{at}purdue.edu (Z.-Q.L.)

This PDF file includes:

  • Fig. S1. Diagrams of the three fic genes present in P. fluorescens strain 2P24.
  • Fig. S2. Alignment of Fic proteins similar to Fic-1 from other bacteria.
  • Fig. S3. Fic-1 affects the DNA yield of plasmids from different incompatibility groups.
  • Fig. S4. Mutants of Fic-2 and Fic-3 defective in the intramolecular inhibitory motif did not affect plasmid DNA yield.
  • Fig. S5. Fic-1 inhibits the growth of both E. coli and P. fluorescens.
  • Fig. S6. Fic-1 AMPylates the N-terminal domain of GyrB (1–200).
  • Fig. S7. Fic-1 AMPylates GyrB from P. fluorescens on Tyr111.
  • Fig. S8. The Fic-1Y5A mutant is defective in self-AMPylation and has low activity against GyrB.
  • Fig. S9. AntF inhibits the activity of Fic-1 by direct interactions.
  • Table S1. Proteins analyzed for interactions with Fic-1.
  • Table S2. Bacterial strains, plasmids, and primers used in the study.
  • Reference (60)

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Citation: C. Lu, E. S. Nakayasu, L.-Q. Zhang, Z.-Q. Luo, Identification of Fic-1 as an enzyme that inhibits bacterial DNA replication by AMPylating GyrB, promoting filament formation. Sci. Signal. 9, ra11 (2016).

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