Supplementary Materials

Supplementary Materials for:

The adhesion GPCR BAI1 mediates macrophage ROS production and microbicidal activity against Gram-negative bacteria

Emily A. Billings, Chang Sup Lee, Katherine A. Owen, Ryan S. D'Souza, Kodi S. Ravichandran, James E. Casanova*

*Corresponding author. E-mail: jec9e{at}virginia.edu

This PDF file includes:

  • Fig. S1. BAI1 localizes to the cell periphery, the perinuclear region, and the sites of bacterial cell association.
  • Fig. S2. BAI1 promotes cellular microbicidal activity in J774 macrophages.
  • Fig. S3. Loss of BAI1 impairs Rac activation in response to E. coli in IFN-γ–primed BMDMs.
  • Fig. S4. Loss of BAI1 impairs intracellular ROS generation but does not affect IFN-γ priming or iNOS production induced by E. coli.
  • Fig. S5. Mitochondrial ROS produced in response to Gram-negative bacteria is independent of BAI1-mediated recognition and signaling.
  • Fig. S6. Measurement of disease activity analysis.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). BAI1 is enriched at the phagocytic cup.

Citation: E. A. Billings, C. S. Lee, K. A. Owen, R. S. D'Souza, K. S. Ravichandran, J. E. Casanova, The adhesion GPCR BAI1 mediates macrophage ROS production and microbicidal activity against Gram-negative bacteria. Sci. Signal. 9, ra14 (2016).

© 2016 American Association for the Advancement of Science