Supplementary Materials

Supplementary Materials for:

Plasma membrane localization of the μ-opioid receptor controls spatiotemporal signaling

Michelle L. Halls,* Holly R. Yeatman, Cameron J. Nowell, Georgina L. Thompson, Arisbel Batista Gondin, Srgjan Civciristov, Nigel W. Bunnett, Nevin A. Lambert, Daniel P. Poole, Meritxell Canals*

*Corresponding author. E-mail: michelle.halls{at}monash.edu (M.L.H.); meri.canals{at}monash.edu (M.C.)

This PDF file includes:

  • Fig. S1. Ligand-dependent spatiotemporal signaling of MOR.
  • Fig. S2. MOR regulation and its effect on cytosolic ERK activity.
  • Fig. S3. Roles of PKC and receptor phosphorylation on the MOR-dependent spatiotemporal profiles of ERK signaling.
  • Fig. S4. MOR localization within the plasma membrane is ligand-dependent.
  • Fig. S5. Effect of membrane disruption on MOR trafficking and spatiotemporal signaling.
  • Table S1. Expression of wild-type MOR and the MOR S375A mutant.

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Citation: M. L. Halls, H. R. Yeatman, C. J. Nowell, G. L. Thompson, A. B. Gondin, S. Civciristov, N. W. Bunnett, N. A. Lambert, D. P. Poole, M. Canals, Plasma membrane localization of the μ-opioid receptor controls spatiotemporal signaling. Sci. Signal. 9, ra16 (2016).

© 2016 American Association for the Advancement of Science