Supplementary Materials

Supplementary Materials for:

Endothelial cells decode VEGF-mediated Ca2+ signaling patterns to produce distinct functional responses

David P. Noren, Wesley H. Chou, Sung Hoon Lee, Amina A. Qutub, Aryeh Warmflash, Daniel S. Wagner, Aleksander S. Popel,* Andre Levchenko*

*Corresponding author. E-mail: andre.levchenko{at}yale.edu (A.L.); apopel{at}jhu.edu (A.S.P.)

This PDF file includes:

  • Text S1. Detailed description of the probabilistic model.
  • Fig. S1. Distribution of averaged Ca2+ concentrations.
  • Fig. S2. Additional analysis of cell motility.
  • Fig. S3. Probabilistic model simulation of responses to spatially uniform VEGF.
  • Fig. S4. Analysis of cell migration in a microfluidic gradient.
  • Fig. S5. Probabilistic model simulation of cells in a gradient of VEGF.
  • Legends for movies S1 to S6

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Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Time-lapse video showing NR, RS, and LP waveforms in PAECs.
  • Movie S2 (.mov format). Time-lapse video showing NR, RS, and LP waveforms in HUVECs.
  • Movie S3 (.avi format). Time-lapse video showing simultaneous Ca2+ and NFAT signaling.
  • Movie S4 (.avi format). Cells exhibiting LP waveforms formed large lamellipodia with pronounced actin translocation.
  • Movie S5 (.avi format). Cells exhibiting RS waveforms displayed only limited actin recruitment and small filopodia.
  • Movie S6 (.avi format). Angiogenic sprout formation in zebrafish.

Citation: D. P. Noren, W. H. Chou, S. H. Lee, A. A. Qutub, A. Warmflash, D. S. Wagner, A. S. Popel, A. Levchenko, Endothelial cells decode VEGF-mediated Ca2+ signaling patterns to produce distinct functional responses. Sci. Signal. 9, ra20 (2016).

© 2016 American Association for the Advancement of Science