Supplementary Materials

Supplementary Materials for:

Dynamic regulation of neutrophil polarity and migration by the heterotrimeric G protein subunits Gαi-GTP and Gβγ

Chinmay R. Surve, Jesi Y. To, Sundeep Malik, Minsoo Kim, Alan V. Smrcka*

*Corresponding author. E-mail: alan_smrcka{at}URMC.rochester.edu

This PDF file includes:

  • Fig. S1. Basal polarization of mouse neutrophils is dependent on ICAM-1.
  • Fig. S2. Activation of Gβγ subunits alone reduces neutrophil motility on an ICAM-1–coated surface.
  • Fig. S3. Uniform activation of Gβγ subunits prevents neutrophil polarity.
  • Fig. S4. GRK2ct blocks the effects of 12155 on cell migration and polarity.
  • Fig. S5. Activation of Gi heterotrimers stimulates neutrophil polarity.
  • Fig. S6. The Gαi1Q204L mutant, but not wild-type Gαi1, prevents cell flattening and enables cell polarization and migration.
  • Fig. S7. Inhibition of PKA restores the polarity of Gβγ-activated cells.
  • Legends for movies S1 to S10

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Activation of Gβγ subunits alone reduces neutrophil motility on an ICAM-1–coated surface.
  • Movie S2 (.mp4 format). Activation of Gi heterotrimers stimulates neutrophil polarity and migration.
  • Movie S3 (.avi format). Activated Gαi does not affect the basal or fMLP-stimulated behavior of HL-60 cells.
  • Movies S4 to S6 (.avi format). Treatment of cells containing activated Gαi with 12155 results in polarized extension of pseudopods, cell movement, and decreased adhesion.
  • Movie S7 (.avi format). Treatment of cells containing wild-type Gαi with 12155 results in cell flattening and depolarization similar to that of untransfected cells.
  • Movie S8 (.mp4 format). Inhibition of PKA restores the polarity of Gβγ-activated cells.
  • Movie S9 (.mp4 format). Inhibition of PKA enhances the basal migration of mouse neutrophils in the absence of 12155.
  • Movie S10 (.mp4 format). Inhibition of PKA does not restore migration in the presence of 12155.

[Download Movies S1 to S10]


Citation: C. R. Surve, J. Y. To, S. Malik, M. Kim, A. V. Smrcka, Dynamic regulation of neutrophil polarity and migration by the heterotrimeric G protein subunits Gαi-GTP and Gβγ. Sci. Signal. 9, ra22 (2016).

© 2016 American Association for the Advancement of Science