Supplementary Materials

Supplementary Materials for:

A calcium-redox feedback loop controls human monocyte immune responses: The role of ORAI Ca2+ channels

Stephanie Saul, Christine S. Gibhardt, Barbara Schmidt, Annette Lis, Bastian Pasieka, David Conrad, Philipp Jung, Rosmarie Gaupp, Bodo Wonnenberg, Ebru Diler, Hedwig Stanisz, Thomas Vogt, Eva C. Schwarz, Markus Bischoff, Mathias Herrmann, Thomas Tschernig, Reinhard Kappl, Heiko Rieger, Barbara A. Niemeyer, Ivan Bogeski*

*Corresponding author. E-mail: ivan.bogeski{at}uks.eu

This PDF file includes:

  • Text S1. ORAI -NOX2 feedback loop: A theoretical model—Detailed description.
  • Fig. S1. ORAI, TRP, and NOX abundance in primary human monocytes.
  • Fig. S2. Transcript and Western blot analysis of ORAI1, STIM1, and STIM2 abundance in primary human monocytes.
  • Fig. S3. ORAI and STIM control monocyte oxidative burst.
  • Fig. S4. The role of ORAI1 in monocyte oxidative burst: Alternative statistical evaluation.
  • Fig. S5. The role of ORAI1, STIM1, and STIM2 in monocyte phagocytosis of uncoated latex beads.
  • Fig. S6. PMA induces increase in ORAI3/ORAI1 ratio in primary human monocytes.
  • Fig. S7. Effect of S. aureus challenge on immune cell recruitment in an acute murine lung infection model.
  • Table S1. siRNA primers.
  • Table S2. Primers used to detect human ORAI, STIM, NOX, and DUOX transcripts and various controls.
  • Table S3. Mouse primers used for qRT-PCR.

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Citation: S. Saul, C. S. Gibhardt, B. Schmidt, A. Lis, B. Pasieka, D. Conrad, P. Jung, R. Gaupp, B. Wonnenberg, E. Diler, H. Stanisz, T. Vogt, E. C. Schwarz, M. Bischoff, M. Herrmann, T. Tschernig, R. Kappl, H. Rieger, B. A. Niemeyer, I. Bogeski, A calcium-redox feedback loop controls human monocyte immune responses: The role of ORAI Ca2+ channels. Sci. Signal. 9, ra26 (2016).

© 2016 American Association for the Advancement of Science