Supplementary Materials

Supplementary Materials for:

A large Rab GTPase encoded by CRACR2A is a component of subsynaptic vesicles that transmit T cell activation signals

Sonal Srikanth,* Kyun-Do Kim, Yuanyuan Gao, Jin Seok Woo, Shubhamoy Ghosh, Guillaume Calmettes, Aviv Paz, Jeff Abramson, Meisheng Jiang, Yousang Gwack*

*Corresponding author. E-mail: ygwack{at}mednet.ucla.edu (Y.G.); ssrikanth{at}mednet.ucla.edu (S.S.)

This PDF file includes:

  • Fig. S1. CRACR2A-a plays a role in SOCE in Jurkat cells.
  • Fig. S2. The C terminus of CRACR2A-a contains a Rab GTPase domain.
  • Fig. S3. Subcellular localization of CRACR2A-a in comparison with Golgi markers and small GTPases.
  • Fig. S4. Subcellular localization of wild-type and mutant CRACR2A-a proteins.
  • Fig. S5. CRACR2A-a is required for the TCR-stimulated phosphorylation of JNK and cell surface expression of CD69.
  • Fig. S6. Genomic organization of human and murine CRACR2A.
  • Fig. S7. CRACR2A deficiency or depletion reduces cytokine production by T cells.
  • Fig. S8. CRACR2A-a colocalizes with STIM1 after store depletion.
  • Fig. S9. Degradation of CRACR2A-a by atorvastatin.
  • Fig. S10. Members of the large GTPase family are defined by their similar domain architectures.
  • Table S1. List of primers and shRNAs used in this study.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Rotation of the 3D projection of endogenous CRACR2A in a Jurkat cell on an anti-CD3 antibody–coated coverslip.
  • Movie S2 (.avi format). TIRF imaging of Lat and CRACR2A-a at the plasma membrane of a spreading Jurkat cell.
  • Movie S3 (.avi format). TIRF imaging of Vav1 and CRACR2A-a at the plasma membrane of a spreading Jurkat cell.
  • Movie S4 (.avi format). TIRF imaging of Vav1 and ΔPRD CRACR2A-a at the plasma membrane of a spreading Jurkat cell.

Citation: S. Srikanth, K.-D. Kim, Y. Gao, J. S. Woo, S. Ghosh, G. Calmettes, A. Paz, J. Abramson, M. Jiang, Y. Gwack, A large Rab GTPase encoded by CRACR2A is a component of subsynaptic vesicles that transmit T cell activation signals. Sci. Signal. 9, ra31 (2016).

© 2016 American Association for the Advancement of Science