Supplementary Materials

Supplementary Materials for:

PLIF: A rapid, accurate method to detect and quantitatively assess protein-lipid interactions

Laurie Ceccato, Gaëtan Chicanne, Virginie Nahoum, Véronique Pons, Bernard Payrastre, Frédérique Gaits-Iacovoni, Julien Viaud*

*Corresponding author. E-mail: julien.viaud{at}inserm.fr

This PDF file includes:

  • Fig. S1. Recombinant proteins used in the study.
  • Fig. S2. Effect of 2% CF-PE and PS on protein recruitment and sensitivity of the PLIF assay.
  • Fig. S3. Characterization of protein domain interactions with PI using liposome flotation.
  • Fig. S4. PLIF assay using wild-type and K32A mutant GST-PH (PLC-δ1).
  • Fig. S5. Protein-PI binding specificity in the presence of 20% PS.
  • Fig. S6. Effect of CF-PE on protein recruitment.
  • Fig. S7. Oligomeric state analysis of GST fusion proteins.
  • Table S1. S/N ratio calculated from fig. S4.
  • Table S2. List of essential reagents used in this study.
  • Table S3. List of lipids used in this study.
  • Table S4. List of main equipment used in this study.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.79 MB


Citation: L. Ceccato, G. Chicanne, V. Nahoum, V. Pons, B. Payrastre, F. Gaits-Iacovoni, J. Viaud, PLIF: A rapid, accurate method to detect and quantitatively assess protein-lipid interactions. Sci. Signal. 9, rs2 (2016).

© 2016 American Association for the Advancement of Science