Supplementary Materials

Supplementary Materials for:

Inhibition of class I histone deacetylases blunts cardiac hypertrophy through TSC2-dependent mTOR repression

Cyndi R. Morales, Dan L. Li, Zully Pedrozo, Herman I. May, Nan Jiang, Viktoriia Kyrychenko, Geoffrey W. Cho, Soo Young Kim, Zhao V. Wang, David Rotter, Beverly A. Rothermel, Jay W. Schneider, Sergio Lavandero, Thomas G. Gillette, Joseph A. Hill*

*Corresponding author. E-mail: joseph.hill{at}utsouthwestern.edu

This PDF file includes:

  • Fig. S1. Effect of class I HDAC inhibition on NRVM hypertrophy and AKT.
  • Fig. S2. Characterization of HDAC inhibition in NRVMs.
  • Fig. S3. Effect of siRNAs targeting class I HDACs on NRVM hypertrophy.
  • Fig. S4. In vivo experimental model.
  • Fig. S5. mTOR and TSC2 are not acetylated in NRVMs treated with HDAC inhibitors.
  • Fig. S6. Inhibition of mTOR by HDAC inhibitors likely depends on transcription.
  • Fig. S7. Real-time PCR screen of components of mTORC1, mTORC2, and the TSC1/TSC2 complexes.
  • Fig. S8. Deptor is not required for HDAC-dependent inhibition of mTOR.
  • Fig. S9. HDAC inhibitor–dependent suppression of mTOR is cell type–specific.
  • Fig. S10. ChIP analysis of the TSC2 promoter.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.29 MB


Citation: C. R. Morales, D. L. Li, Z. Pedrozo, H. I. May, N. Jiang, V. Kyrychenko, G. W. Cho, S. Y. Kim, Z. V. Wang, D. Rotter, B. A. Rothermel, J. W. Schneider, S. Lavandero, T. G. Gillette, J. A. Hill, Inhibition of class I histone deacetylases blunts cardiac hypertrophy through TSC2-dependent mTOR repression. Sci. Signal. 9, ra34 (2016).

© 2016 American Association for the Advancement of Science