Supplementary Materials

Supplementary Materials for:

Identification of GPR83 as the receptor for the neuroendocrine peptide PEN

Ivone Gomes, Erin N. Bobeck, Elyssa B. Margolis, Achla Gupta, Salvador Sierra, Amanda K. Fakira, Wakako Fujita, Timo D. Müller, Anne Müller, Matthias H. Tschöp, Gunnar Kleinau, Lloyd D. Fricker, Lakshmi A. Devi*

*Corresponding author. Email: lakshmi.devi{at}mssm.edu

This PDF file includes:

  • Fig. S1. Variable results with studies examining the effect of mPEN on adenylyl cyclase activity in hypothalamic membranes.
  • Fig. S2. The binding of mPEN to hypothalamic membranes from individual male mice.
  • Fig. S3. Variable results with studies examining the effect of mPEN on PLC activity in hippocampal membranes.
  • Fig. S4. Evoked EPSC amplitude after sequential application of increasing concentrations of mPEN and washout.
  • Fig. S5. mPEN-stimulated neurite outgrowth in Neuro2A cells.
  • Fig. S6. Expression of GPR83 in heterologous cells confers PEN signaling and receptor endocytosis.
  • Fig. S7. Quantitative RT-PCR to confirm the presence of GPR83 mRNA in Neuro2A cells.
  • Fig. S8. Specificity of the GPR83 and GPR171 antibodies.
  • Fig. S9. Confirmation of antibody specificity and accuracy of colocalization by analysis of the lateral septum.
  • Table S1. Description of statistical analysis for different figures.

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Citation: I. Gomes, E. N. Bobeck, E. B. Margolis, A. Gupta, S. Sierra, A. K. Fakira, W. Fujita, T. D. Müller, A. Müller, M. H. Tschöp, G. Kleinau, L. D. Fricker, L. A. Devi, Identification of GPR83 as the receptor for the neuroendocrine peptide PEN. Sci. Signal. 9, ra43 (2016).

© 2016 American Association for the Advancement of Science