Supplementary Materials

Supplementary Materials for:

Themis1 enhances T cell receptor signaling during thymocyte development by promoting Vav1 activity and Grb2 stability

Ekaterina Zvezdova, Judith Mikolajczak, Anne Garreau, Marlène Marcellin, Lise Rigal, Jan Lee, Seeyoung Choi, Gaëtan Blaize, Jérémy Argenty, Julien Familiades, Liqi Li, Anne Gonzalez de Peredo, Odile Burlet-Schiltz, Paul E. Love, Renaud Lesourne*

*Corresponding author. Email: renaud.lesourne{at}inserm.fr

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Themis1 enhances TCR signaling during thymic selection.
  • Fig. S2. Analysis of the interaction between Themis1 and Vav1 in thymocytes and HEK 293T cells.
  • Fig. S3. Themis1−/− thymocytes exhibit normal phosphorylation of Lck, ZAP-70, and LAT after cross-linking of the TCR with low or high concentrations of antibody complexes.
  • Fig. S4. The amount of Grb2 that coimmunoprecipitates with c-Cbl and Sos1 is reduced in thymocytes from Themis1−/− mice.
  • Fig. S5. Loss of c-Cbl does not affect Grb2 protein abundance in thymocytes.
  • Fig. S6. Analysis of TCR signaling and Grb2 coimmunoprecipitation with Vav1 and LAT in Themis1 transgenic thymocytes.
  • Fig. S7. Effect of Themis1 overexpression on T cell development.
  • Fig. S8. Transgenic expression of Themis1 in Grb2+/− mice restores positive selection in TCR transgenic mice.
  • Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of proteins identified by proteomic analysis of Themis1-immunopurified samples.

Citation: E. Zvezdova, J. Mikolajczak, A. Garreau, M. Marcellin, L. Rigal, J. Lee, S. Choi, G. Blaize, J. Argenty, J. Familiades, L. Li, A. Gonzalez de Peredo, O. Burlet-Schiltz, P. E. Love, R. Lesourne, Themis1 enhances T cell receptor signaling during thymocyte development by promoting Vav1 activity and Grb2 stability. Sci. Signal. 9, ra51 (2016).

© 2016 American Association for the Advancement of Science