Supplementary Materials

Supplementary Materials for:

Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3–induced outside-in signaling and clusterin release

Lili Donner, Knut Fälker, Lothar Gremer, Stefan Klinker, Giulia Pagani, Liza U. Ljungberg, Kimberley Lothmann, Federica Rizzi, Martin Schaller, Holger Gohlke, Dieter Willbold, Magnus Grenegard, Margitta Elvers*

*Corresponding author. Email: margitta.elvers{at}med.uni-duesseldorf.de

This PDF file includes:

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  • Fig. S1. Characterization of platelet-mediated Aβ aggregate formation in culture.
  • Fig. S2. CLU promotes platelet-mediated Aβ aggregate formation in culture.
  • Fig. S3. Flow cytometric analysis confirmed Aβ binding to integrin αIIbβ3.
  • Fig. S4. Congo red staining of Aβ aggregate formation.
  • Fig. S5. Aβ-induced aggregation of and ATP release from human platelets.
  • Fig. S6. Structural stability of domains in integrin αIIbβ3.
  • Fig. S7. Structural stability of Aβ peptides bound to integrin αIIbβ3.
  • Fig. S8. Hydrogen bond formation of Nε of His6 of Aβ40 with the carbonyl group of Asn215.
  • Fig. S9. Tirofiban treatment of mice is not sufficient because platelet inhibition is temporary.
  • References (8294)

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Citation: L. Donner, K. Fälker, L. Gremer, S. Klinker, G. Pagani, L. U. Ljungberg, K. Lothmann, F. Rizzi, M. Schaller, H. Gohlke, D. Willbold, M. Grenegard, M. Elvers, Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3–induced outside-in signaling and clusterin release. Sci. Signal. 9, ra52 (2016).

© 2016 American Association for the Advancement of Science