Supplementary Materials

Supplementary Materials for:

Design of pathway preferential estrogens that provide beneficial metabolic and vascular effects without stimulating reproductive tissues

Zeynep Madak-Erdogan, Sung Hoon Kim, Ping Gong, Yiru C. Zhao, Hui Zhang, Ken L. Chambliss, Kathryn E. Carlson, Christopher G. Mayne, Philip W. Shaul, Kenneth S. Korach, John A. Katzenellenbogen, Benita S. Katzenellenbogen*

*Corresponding author. Email: katzenel{at}illinois.edu

This PDF file includes:

  • Fig. S1. ER and coactivator binding and interaction assays with ligands and ligand dissociation rates.
  • Fig. S2. Verification of siRNA results in Fig. 2C with another siRNA.
  • Fig. S3. PLAs with E2 and PaPE-1.
  • Fig. S4. Pharmacokinetic studies for analysis of blood concentrations of PaPE-1 after injection or pellet implantation.
  • Fig. S5. Effects of PaPE-1 require ERα.
  • Legends for tables S1 and S2

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Technical Details

Format: Adobe Acrobat PDF

Size: 1.46 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of genes differentially expressed by cell treatment with E2 and PaPE-1.
  • Table S2 (Microsoft Excel format). BED files for ERα, ERK2, and pSer5 RNA pol II ChIP-seq data from experiments with MCF-7 cells.

Citation: Z. Madak-Erdogan, S. H. Kim, P. Gong, Y. C. Zhao, H. Zhang, K. L. Chambliss, K. E. Carlson, C. G. Mayne, P. W. Shaul, K. S. Korach, J. A. Katzenellenbogen, B. S. Katzenellenbogen, Design of pathway preferential estrogens that provide beneficial metabolic and vascular effects without stimulating reproductive tissues. Sci. Signal. 9, ra53 (2016).

© 2016 American Association for the Advancement of Science