Supplementary Materials for:

The 4E-BP–eIF4E axis promotes rapamycin-sensitive growth and proliferation in lymphocytes

Lomon So, Jongdae Lee, Miguel Palafox, Sharmila Mallya, Chaz G. Woxland, Meztli Arguello, Morgan L. Truitt, Nahum Sonenberg, Davide Ruggero, David A. Fruman*

*Corresponding author. Email: dfruman{at}uci.edu

This PDF file includes:

• Fig. S1. Acute inhibition of S6K activity is dispensable for lymphocyte growth and proliferation.
• Fig. S2. Hypomorphic genetic model shows that S6K activity is dispensable for lymphocyte growth and proliferation.
• Fig. S3. Specific inactivation of mTOR kinase activity in lymphocytes phenocopies complete mTOR or mTORC1 loss in lymphocytes.
• Fig. S4. Amino acid sequence surrounding the Thr³⁷ phosphoacceptor site on 4E-BP1 and 4E-BP2 is distinct and conserved.
• Fig. S5. Gene expression patterns of eif4ebp1 and eif4ebp2 are distinct specifically in mature lymphocytes.
• Fig. S6. Rapamycin partially inhibits eIF4F complex formation in lymphoma cells and MEFs.
• Fig. S7. Rapamycin has a lesser effect on the proliferation of MEFs than that of MLN0128.
• Fig. S8. 4E-BP1^M can be inducibly expressed in naïve lymphocytes and is sufficient to block growth and proliferation equivalent to rapamycin or TOR-KIs.
• Fig. S9. 4E-BP1^M blocks lymphocyte growth and proliferation in vivo.
• Fig. S10. 4E-BP1^M inhibits only proliferation without affecting size in lymphoma cells.
• Table S1. Accession numbers for species analyzed.

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Citation: L. So, J. Lee, M. Palafox, S. Mallya, C. G. Woxland, M. Arguello, M. L. Truitt, N. Sonenberg, D. Ruggero, D. A. Fruman, The 4E-BP–eIF4E axis promotes rapamycin-sensitive growth and proliferation in lymphocytes. Sci. Signal. 9, ra57 (2016).

© 2016 American Association for the Advancement of Science