Supplementary Materials

Supplementary Materials for:

A switch in G protein coupling for type 1 corticotropin-releasing factor receptors promotes excitability in epileptic brains

Chakravarthi Narla, Tanner Scidmore, Jaymin Jeong, Michelle Everest, Peter Chidiac, Michael O. Poulter*

*Corresponding author. Email: mpoulter{at}robarts.ca

This PDF file includes:

  • Fig. S1. No changes in CRFR1, CRFR2, or CRF immunoreactivity in the layers of the piriform cortex.
  • Fig. S2. The expression of the mRNAs encoding Gαs and Gαq/11 is unchanged after kindling.
  • Fig. S3. qPCR analysis of mRNAs encoding proteins involved in GPCR signaling.
  • Fig. S4. Kindling-induced changes in the phosphorylation of signaling proteins.
  • Fig. S5. CRFR2 antagonism did not block the effects of CRF.
  • Fig. S6. PKC or PKA antagonism did not affect CRFR1 activation in wild-type and RGS2 KO mouse, respectively.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Excitation and disinhibition in the layers of nonkindled rat piriform cortex imaged through VSDI.
  • Movie S2 (.avi format). Change in the excitation and disinhibition in the layers of nonkindled rat piriform cortex after CRF application.
  • Movie S3 (.avi format). Excitation and disinhibition in the layers of kindled rat piriform cortex imaged through VSDI.
  • Movie S4 (.avi format). Change in the excitation and disinhibition in the layers of kindled rat piriform cortex after CRF application.

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Citation: C. Narla, T. Scidmore, J. Jeong, M. Everest, P. Chidiac, M. O. Poulter, A switch in G protein coupling for type 1 corticotropin-releasing factor receptors promotes excitability in epileptic brains. Sci. Signal. 9, ra60 (2016).

© 2016 American Association for the Advancement of Science