Supplementary Materials

Supplementary Materials for:

Proximity biotinylation provides insight into the molecular composition of focal adhesions at the nanometer scale

Jing-Ming Dong, Felicia Pei-Ling Tay, Hannah Lee-Foon Swa, Jayantha Gunaratne, Thomas Leung, Brian Burke, Ed Manser*

*Corresponding author. Email: ed.manser{at}imcb.a-star.edu.sg

This PDF file includes:

  • Fig. S1. Characterization of U2OS cell lines stably expressing GFP-BirA*-paxillin.
  • Fig. S2. Intracellular localization of some GFP-tagged proteins that were identified through GFP-BirA*-paxillin SILAC BioID.
  • Fig. S3. The SILAC H/L ratio is not related to its protein abundance in the cell.
  • Fig. S4. Identification of paxillin-associated proteins by AP-MS.
  • Fig. S5. Colocalization of paxillin and kindlin-2 in U2OS cells.
  • Table S1. SILAC-enriched paxillin-associated proteins identified by AP-MS.
  • Table S2. List of proteins identified by GFP–BirA*–kindlin-2 SILAC BioID.
  • Table S3. Summary of reported blebbistatin sensitivity of paxillin-proximal proteins based on MS analysis of fibroblast proteins.
  • Legend for data file S1

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Unfiltered data sets of protein identified by paxillin and kindlin-2 BioID.

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Citation: J.-M. Dong, F. P.-L. Tay, H. L.-F. Swa, J. Gunaratne, T. Leung, B. Burke, E. Manser, Proximity biotinylation provides insight into the molecular composition of focal adhesions at the nanometer scale. Sci. Signal. 9, rs4 (2016).

© 2016 American Association for the Advancement of Science