Supplementary Materials

Supplementary Materials for:

Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging

Kareem Elsayad,* Stephanie Werner, Marçal Gallemí, Jixiang Kong, Edmundo R. Sánchez Guajardo, Lijuan Zhang, Yvon Jaillais, Thomas Greb, Youssef Belkhadir*

*Corresponding author. Email: kareem.elsayad{at}vbcf.ac.at (K.E.); youssef.belkhadir{at}gmi.oeaw.ac.at (Y.B.)

This PDF file includes:

  • Note S1. Details of FBi.
  • Note S2. Effect of anisotropy in n for calculated M′.
  • Note S3. Effect of uncertainty in n and ρ for calculated M′.
  • Note S4. Experimental determination of the refractive index and density of Arabidopsis ECMs.
  • Fig. S1. Schematic of the FBi setup.
  • Fig. S2. Sketch of the used microscope and spectrometer setup.
  • Fig. S3. FBi scans of epidermal onion cells.
  • Fig. S4. FBi scans of Lti6-tdTomato root cells.
  • Fig. S5. Width measurements of Arabidopsis hypocotyl longitudinal ECMs.
  • Fig. S6. Effect of plasmolysis on ECM and nearby cytoplasm.
  • Fig. S7. Determination of refractive index and density of Arabidopsis ECM by timeresolved fluorescence studies.
  • Fig. S8. Cross-sectional Brillouin imaging scans of cells in deep layers of Arabidopsis roots.
  • Fig. S9. Cross-sectional FBi scans of wild-type Arabidopsis root cells.
  • Reference (69)

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.3 MB


Citation: K. Elsayad, S. Werner, M. Gallemí, J. Kong, E. R. Sánchez Guajardo, L. Zhang, Y. Jaillais, T. Greb, Y. Belkhadir, Mapping the subcellular mechanical properties of live cells in tissues with fluorescence emission–Brillouin imaging. Sci. Signal. 9, rs5 (2016).

© 2016 American Association for the Advancement of Science