Supplementary Materials

Supplementary Materials for:

ROCK2 signaling is required to induce a subset of T follicular helper cells through opposing effects on STATs in autoimmune settings

Jonathan M. Weiss, Wei Chen, Melanie S. Nyuydzefe, Alissa Trzeciak, Ryan Flynn, James R. Tonra, Suzana Marusic, Bruce R. Blazar, Samuel D. Waksal, Alexandra Zanin-Zhorov*

*Corresponding author. Email: alexandra.zanin-zhorov{at}kadmon.com

This PDF file includes:

  • Fig. S1. The TH17 cell–like skewing of human CD4+ T cells induces a TFH cell–like phenotype.
  • Fig. S2. ROCK2 is required for the generation and function of human TFH cells.
  • Fig. S3. KD025 decreases the percentage of CXCR5+PD1+ T cells in the presence of IL-21 in vitro.
  • Fig. S4. STAT3 is required for Bcl6 and Blimp1 in human TFH cells.
  • Fig. S5. KD025 stops the progression of kidney damage in MRL/lpr mice.
  • Fig. S6. KD025 inhibits IL-17 and IL-21 secretion by stimulated PBMCs from active SLE patients.
  • Fig. S7. KD025 oppositely regulates STAT3 and STAT5 binding to the Il21 promoter.
  • Fig. S8. Pharmacokinetic analysis of MRL/lpr mice treated with KD025.

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Citation: J. M. Weiss, W. Chen, M. S. Nyuydzefe, A. Trzeciak, R. Flynn, J. R. Tonra, S. Marusic, B. R. Blazar, S. D. Waksal, A. Zanin-Zhorov, ROCK2 signaling is required to induce a subset of T follicular helper cells through opposing effects on STATs in autoimmune settings. Sci. Signal. 9, ra73 (2016).

© 2016 American Association for the Advancement of Science