Supplementary Materials

Supplementary Materials for:

Synergistically acting agonists and antagonists of G protein–coupled receptors prevent photoreceptor cell degeneration

Yu Chen,* Grazyna Palczewska, Ikuo Masuho, Songqi Gao, Hui Jin, Zhiqian Dong, Linn Gieser, Matthew J. Brooks, Philip D. Kiser, Timothy S. Kern, Kirill A. Martemyanov, Anand Swaroop, Krzysztof Palczewski*

*Corresponding author. Email: chenyu6639{at}hotmail.com (Y.C.); kxp65{at}case.edu (K.P.)

This PDF file includes:

  • Fig. S1. Abca4−/−Rdh8−/− mice exposed to bright light exhibit pyknosis of photoreceptor cells, diminished synaptophysin in the OPL, and altered horizontal cell morphology.
  • Fig. S2. Pharmacological pretreatments targeting different GPCRs preserve retinal structure in bright light–exposed Abca4−/−Rdh8−/− mice.
  • Fig. S3. Variable preservation of retinal morphology by pharmacological pretreatments targeting different GPCRs in bright light–exposed Abca4−/−Rdh8−/− mice.
  • Fig. S4. Pharmacological pretreatment affecting various GPCRs preserves retinal function in bright light–exposed Abca4−/−Rdh8−/− mice.
  • Fig. S5. BRM or MTP pretreatment protects retinas of BALB/c mice from bright light–induced degeneration.
  • Fig. S6. Transcriptome analysis of the retina.
  • Fig. S7. Dose-dependent protection of retinal morphology in Abca4−/−Rdh8−/− mice by BRM, MTP, and TAM pretreatment.
  • Fig. S8. Combined pretreatments improve retinal morphological protection against bright light–exposed Abca4−/−Rdh8−/− mice.
  • Fig. S9. Combined treatments with subeffective doses of individual drugs exhibit improved retinal morphological protection in bright light–exposed Abca4−/−Rdh8−/− mice.
  • Fig. S10. Combined pretreatments protect retinas of BALB/c mice from bright light–induced photoreceptor degeneration.
  • Table S1. Retinal gene sets significantly altered in response to bright light exposure in Abca4−/−Rdh8−/− mice.
  • Table S2. Retinal gene sets beneficially regulated by BRM pretreatment.
  • Table S3. Retinal gene sets beneficially affected by MTP pretreatment.
  • Table S4. Retinal gene sets beneficially affected by TAM pretreatment.
  • Table S5. Retinal gene sets beneficially affected by DOX pretreatment.
  • Table S6. Significantly altered retinal gene sets as a result of bright light exposure in Abca4−/−Rdh8−/− mice.
  • Table S7. Retinal gene sets beneficially affected as a result of combined pretreatment with BRM, MTP, and TAM.
  • Table S8. Retinal gene sets beneficially affected as a result of combined pretreatment with BRM, MTP, and DOX.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.70 MB


Citation: Y. Chen, G. Palczewska, I. Masuho, S. Gao, H. Jin, Z. Dong, L. Gieser, M. J. Brooks, P. D. Kiser, T. S. Kern, K. A. Martemyanov, A. Swaroop, K. Palczewski, Synergistically acting agonists and antagonists of G protein–coupled receptors prevent photoreceptor cell degeneration. Sci. Signal. 9, ra74 (2016).

© 2016 American Association for the Advancement of Science