Supplementary Materials

Supplementary Materials for:

Small heterodimer partner SHP mediates liver X receptor (LXR)–dependent suppression of inflammatory signaling by promoting LXR SUMOylation specifically in astrocytes

Jee Hoon Lee, Hyunmi Kim, Soo Jung Park, Joo Hong Woo, Eun-hye Joe, Ilo Jou*

*Corresponding author. Email: jouilo{at}aumc.ac.kr

This PDF file includes:

  • Fig. S1. LXRs interact with SHP and induce expression of SHP in a receptor-dependent manner.
  • Fig. S2. SHP is involved in LXR-mediated signaling pathways in brain astrocytes.
  • Fig. S3. SHP contributes to LXR-mediated anti-inflammatory effects in brain astrocytes.
  • Fig. S4. Interactions between SHP and LXR are critical for LXR-mediated anti-inflammatory effects.
  • Fig. S5. SUMOylation mediates the LXR-dependent anti-inflammatory effect.
  • Fig. S6. SHP promotes LXR SUMOylation.
  • Fig. S7. SHP promotes PIAS1 protein stability.
  • Fig. S8. SHP promotes PIAS1-mediated LXRβ SUMOylation by inhibiting SIAH1 binding to PIAS1.
  • Table S1. List of siRNA oligonucleotides.
  • Legend for data file S1

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.62 MB

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Gene expression analysis of brain astrocytes upon SHP knockdown.

[Download Data file S1]


Citation: J. H. Lee, H. Kim, S. J. Park, J. H. Woo, E.-h. Joe, I. Jou, Small heterodimer partner SHP mediates liver X receptor (LXR)–dependent suppression of inflammatory signaling by promoting LXR SUMOylation specifically in astrocytes. Sci. Signal. 9, ra78 (2016).

© 2016 American Association for the Advancement of Science