Supplementary Materials

Supplementary Materials for:

Multiplex matrix network analysis of protein complexes in the human TCR signalosome

Stephen E. P. Smith, Steven C. Neier, Brendan K. Reed, Tessa R. Davis, Jason P. Sinnwell, Jeanette E. Eckel-Passow, Gabriel F. Sciallis, Carilyn N. Wieland, Rochelle R. Torgerson, Diana Gil, Claudia Neuhauser,* Adam G. Schrum*

*Corresponding author. Email: schrum.adam{at}mayo.edu (A.G.S.); neuha001{at}umn.edu (C.N.)

This PDF file includes:

  • Equations
  • Fig. S1. Screening strategy for multiplex panel antibodies, using SLP-76 as an example.
  • Fig. S2. Instrument setup for optimal analysis of protein complexes.
  • Fig. S3. Development and evaluation of ANC analysis.
  • Fig. S4. Application and evaluation of WCNA analysis for the SEE-stimulated and unstimulated Jurkat cell data.
  • Fig. S5. Stimulation-induced PiSCES network is similar between control and alopecia areata patient groups.
  • Table S1. Validated antibody pairs used to identify each target in Jurkat cells.
  • Table S2. Phenotypic characteristics of alopecia areata patients and controls and their T cell populations.

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Citation: S. E. P. Smith, S. C. Neier, B. K. Reed, T. R. Davis, J. P. Sinnwell, J. E. Eckel-Passow, G. F. Sciallis, C. N. Wieland, R. R. Torgerson, D. Gil, C. Neuhauser, A. G. Schrum, Multiplex matrix network analysis of protein complexes in the human TCR signalosome. Sci. Signal. 9, rs7 (2016).

© 2016 American Association for the Advancement of Science