Supplementary Materials

Supplementary Materials for:

Histone deacetylase 3 supports endochondral bone formation by controlling cytokine signaling and matrix remodeling

Lomeli R. Carpio, Elizabeth W. Bradley, Meghan E. McGee-Lawrence, Megan M. Weivoda, Daniel D. Poston, Amel Dudakovic, Ming Xu, Tamar Tchkonia, James L. Kirkland, Andre J. van Wijnen, Merry Jo Oursler, Jennifer J. Westendorf*

*Corresponding author. Email: westendorf.jennifer{at}mayo.edu

This PDF file includes:

  • Fig. S1. Expression pattern of Hdac3 during embryonic and postnatal long bone development.
  • Fig. S2. Hdac3 knockout in chondrocytes during embryogenesis is lethal.
  • Fig. S3. Tamoxifen does not affect growth plate maturation in wild-type mice.
  • Fig. S4. Top 30 annotation clusters of genes with decreased gene expression in Hdac3-depleted IMCs.
  • Fig. S5. Top 30 annotation clusters of genes with increased gene expression in Hdac3-depleted IMCs.
  • Fig. S6. Gene lists associated with top annotation clusters from DAVID pathway analysis.
  • Fig. S7. GSEA on differentially expressed genes in Hdac3-depleted IMCs.
  • Fig. S8. JAK Inhibitor I reduces STAT3 phosphorylation and Il-6 and Mmp13 gene expression in a concentration-dependent manner in Hdac3-depleted chondrocytes.
  • Fig. S9. JAK-STAT pathway inhibitors reduce STAT3 phosphorylation and Il-6 and Mmp13 gene expression in Hdac3-depleted chondrocytes.
  • Fig. S10. Combining JAK-STAT pathway and NF-κB inhibitors does not further reduce Il-6 and Mmp13 gene expression in Hdac3-depleted chondrocytes.
  • Fig. S11. The Col2a1ERT-Cre driver is expressed in chondrocytes and osteoblasts but does not increase IL-6 abundance in osteoblasts.
  • Fig. S12. JAK inhibition partially restores the bone phenotype of Hdac3-CKOCol2ERT mice.
  • Table S1. Primer sequences.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.30 MB


Citation: L. R. Carpio, E. W. Bradley, M. E. McGee-Lawrence, M. M. Weivoda, D. D. Poston, A. Dudakovic, M. Xu, T. Tchkonia, J. L. Kirkland, A. J. van Wijnen, M. J. Oursler, J. J. Westendorf, Histone deacetylase 3 supports endochondral bone formation by controlling cytokine signaling and matrix remodeling. Sci. Signal. 9, ra79 (2016).

© 2016 American Association for the Advancement of Science