Supplementary Materials

Supplementary Materials for:

Ras and TGF-β signaling enhance cancer progression by promoting the ΔNp63 transcriptional program

Eleftheria Vasilaki, Masato Morikawa, Daizo Koinuma, Anna Mizutani, Yudai Hirano, Shogo Ehata, Anders Sundqvist, Natsumi Kawasaki, Jessica Cedervall, Anna-Karin Olsson, Hiroyuki Aburatani, Aristidis Moustakas, Kohei Miyazono,* Carl-Henrik Heldin*

*Corresponding authors. Emails: miyazono{at}m.u-tokyo.ac.jp; c-h.heldin{at}licr.uu.se

This PDF file includes:

  • Fig. S1. Knockdown of the ΔN isoforms of p63 results in the same effects as knockdown of all p63 isoforms.
  • Fig. S2. p63 is necessary for the up-regulation of DUSP6 and DUSP7 by Ras and TGF-β signaling.
  • Fig. S3. ΔNp63 and its target genes, DUSP6 and DUSP7, promote metastatic behavior in cultured cancer cells.
  • Fig. S4. Correlation of mutant status of p53 and expression of TP63 with survival in cancer patients.
  • Fig. S5. Stability of p53-R273H protein and its phosphorylation site mutants.
  • Table S1. List of all the genes in subgroup 1 (p63 as an activator).
  • Table S2. List of all the genes in subgroup 2 (p63 as a repressor).
  • Table S3. Primer sequences used for ChIP-qPCR and qRT-PCR.
  • Table S4. Primer sequences used for cloning and mutagenesis.

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Citation: E. Vasilaki, M. Morikawa, D. Koinuma, A. Mizutani, Y. Hirano, S. Ehata, A. Sundqvist, N. Kawasaki, J. Cedervall, A.-K. Olsson, H. Aburatani, A. Moustakas, K. Miyazono, C.-H. Heldin, Ras and TGF-β signaling enhance cancer progression by promoting the ΔNp63 transcriptional program. Sci. Signal. 9, ra84 (2016).

© 2016 American Association for the Advancement of Science