Supplementary Materials

Supplementary Materials for:

Negative regulation of NF-κB p65 activity by serine 536 phosphorylation

Jean-Philippe Pradère, Céline Hernandez, Christiane Koppe, Richard A. Friedman, Tom Luedde, Robert F. Schwabe*

*Corresponding author. Email: rfs2102{at}cumc.columbia.edu

This PDF file includes:

  • Fig. S1. Phenotypic characterization of S534A knock-in mice.
  • Fig. S2. Determination of NF-κB activation in MEFs, spleens, and livers from wildtype and S534A mice.
  • Fig. S3. Analysis of gene expression in the livers of wild-type and S534A mice injected with low-dose LPS.
  • Fig. S4. Expression of NF-κB–dependent genes in the spleens of wild-type and S534A mice at early and late time points after injection with low-dose LPS.
  • Fig. S5. Analysis of p65 protein abundance and NF-κB target gene expression in macrophages from wild-type and S534A mice.
  • Fig. S6. IKK phosphorylates p65 at multiple sites.
  • Table S1. Complete blood counts in wild-type and S534A mice.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.1 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Hepatic gene expression profiles in untreated wild-type and S534A mice.
  • Table S3 (Microsoft Excel format). Hepatic gene expression profiles in wild-type and S534A mice treated with high-dose LPS.
  • Table S4 (Microsoft Excel format). Hepatic gene expression profiles in wild-type and S534A mice treated with low-dose LPS.

[Download Tables S2 to S4]


Citation: J.-P. Pradère, C. Hernandez, C. Koppe, R. A. Friedman, T. Luedde, R. F. Schwabe, Negative regulation of NF-κB p65 activity by serine 536 phosphorylation. Sci. Signal. 9, ra85 (2016).

© 2016 American Association for the Advancement of Science