Supplementary Materials for:

Proteome-wide analysis of arginine monomethylation reveals widespread occurrence in human cells

Sara C. Larsen, Kathrine B. Sylvestersen, Andreas Mund, David Lyon, Meeli Mullari, Maria V. Madsen, Jeremy A. Daniel, Lars J. Jensen, Michael L. Nielsen*

*Corresponding author. Email: michael.lund.nielsen{at}cpr.ku.dk

This PDF file includes:

• Supplementary note on mass spectrometric identification of arginine-methylated peptides
• Fig. S1. Validation of established methodology for mapping arginine methylation sites.
• Fig. S2. Arginine methylation sites are found in major protein complexes.
• Fig. S3. Site-specific analysis of arginine methylation sites.
• Fig. S4. Occupancy analysis of arginine methylation sites.
• Fig. S5. High-content microscopy analysis of splicing component SRSF2.
• Fig. S6. Cell cycle analysis using QIBC.
• Reference (81)

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). List of identified arginine methylation sites from all experiments.
• Table S2 (Microsoft Excel format). List of protein complexes found significantly
  enriched in arginine methylation protein factors.
• Table S3 (Microsoft Excel format). List of Pfam domains where arginine
  methylation sites occur.
• Table S4 (Microsoft Excel format). Stoichiometry values for identified arginine
  methylation sites.
• Table S5. List of identified proteins used for determination of arginine methylation stoichiometry values.

[Download Tables S1 to S5]

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Citation: S.C. Larsen, K.B. Sylvestersen, A. Mund, D. Lyon, M. Mullari, M.V. Madsen, J.A. Daniel, L.J. Jensen, M.L. Nielsen. Sci. Signal. 9, rs9 (2016).

© 2016 American Association for the Advancement of Science