Supplementary Materials

Supplementary Materials for:

MuSK is a BMP co-receptor that shapes BMP responses and calcium signaling in muscle cells

Atilgan Yilmaz, Chandramohan Kattamuri, Rana N. Ozdeslik, Carolyn Schmiedel, Sarah Mentzer, Christoph Schorl, Elena Oancea, Thomas B. Thompson, Justin R. Fallon*

*Corresponding author. Email: justin_fallon{at}brown.edu

This PDF file includes:

  • Fig. S1. SPR binding analysis of MuSK with BMP2 and BMP7.
  • Fig. S2. Soluble MuSK ectodomain inhibits BMP4 activity.
  • Fig. S3. Equivalent binding of biglycan to MuSK ectodomain either containing (FL) or lacking (ΔIg3) the third Ig domain.
  • Fig. S4. BMP4 treatment stimulates Rgs4 expression in wild-type but not MuSK−/− myoblasts.
  • Fig. S5. MuSK favors the transcription of signaling- and transcription-related genes.
  • Fig. S6. Abundant phosphorylated SMAD1/5/8 in cytosolic granules in wild-type but not MuSK−/− myoblasts.
  • Table S1. Association and dissociation rate constants and overall Kd values for the interaction of MuSK with BMP2, BMP4, and BMP7, as determined by SPR.
  • Table S2. Transcripts induced by BMP4 only in wild-type myoblasts.
  • Table S3. Transcripts induced by BMP4 in both wild-type and MuSK−/− myoblasts.
  • Table S4. Transcripts induced by BMP4 only in MuSK−/− myoblasts.
  • Table S5. Transcripts induced by BMP4 only in wild-type myotubes.
  • Table S6. Transcripts induced by BMP4 in both wild-type and MuSK−/− myotubes.
  • Table S7. Transcripts induced by BMP4 only in MuSK−/− myotubes.
  • Table S8. Transcripts induced by BMP4 in a MuSK-dependent manner in both myoblasts and myotubes.
  • Table S9. Transcripts induced by BMP4 in a MuSK-dependent manner only in myoblasts.
  • Table S10. Transcripts induced by BMP4 in a MuSK-dependent manner only in myotubes.

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Citation: A. Yilmaz, C. Kattamuri, R. N. Ozdeslik, C. Schmiedel, S. Mentzer, C. Schorl, E. Oancea, T. B. Thompson, J. R. Fallon, MuSK is a BMP co-receptor that shapes BMP responses and calcium signaling in muscle cells. Sci. Signal. 9, ra87 (2016).

© 2016 American Association for the Advancement of Science