Supplementary Materials

Supplementary Materials for:

Chemical proteomic map of dimethyl fumarate–sensitive cysteines in primary human T cells

Megan M. Blewett, Jiji Xie, Balyn W. Zaro, Keriann M. Backus, Amnon Altman, John R. Teijaro,* Benjamin F. Cravatt*

*Corresponding author. Email: teijaro{at}scripps.edu (J.R.T.); cravatt{at}scripps.edu (B.F.C.)

This PDF file includes:

  • Fig. S1. DMF does not affect T cell viability.
  • Fig. S2. DMF, but not MMF, inhibits the activation of primary mouse T cells.
  • Fig. S3. The cumulative number of unique quantified peptides and proteins after five biological replicates of isoTOP-ABPP.
  • Fig. S4. Analysis of BSO-treated primary human T cells by isoTOP-ABPP.
  • Fig. S5. DMF inhibits the translocation of NF-κB p65 to the nucleus in primary human T cells.
  • Fig. S6. Analysis of the sensitivity of PKCθ residues Cys14 and Cys17 to DMF and MMF.
  • Fig. S7. Densitometric analysis of PKCθ band intensities.
  • Fig. S8. DMF-sensitive cysteine residues in ADA.
  • Table S1. List of DMF-sensitive cysteine residues in human T cells.
  • References (4871)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Complete proteomics data for cysteine residues quantified by isoTOP-ABPP.

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Citation: M. M. Blewett, J. Xie, B. W. Zaro, K. M. Backus, A. Altman, J. R. Teijaro, B. F. Cravatt, Chemical proteomic map of dimethyl fumarate–sensitive cysteines in primary human T cells. Sci. Signal. 9, rs10 (2016).

© 2016 American Association for the Advancement of Science