Supplementary Materials for:

Human IL-22 binding protein isoforms act as a rheostat for IL-22 signaling

Chrissie Lim, MeeAe Hong, Ram Savan*

*Corresponding author. Email: savanram{at}uw.edu

This PDF file includes:

• Fig. S1. Alignment of the amino acid sequences of IL-22BPi1, IL-22BPi2, and IL-22BPi3.
• Fig. S2. Analysis of IL-22BP isoform abundance in pSTAT3 Western blotting assays and optimization of the IL-22 concentration used for STAT3 luciferase reporter assays.
• Fig. S3. Analysis of the efficiency and specificity of the primers and probes used for the qPCR-based analysis of IL22RA2 isoforms.
• Fig. S4. Correlation between the expression of genes encoding membrane-bound IL-22R subunits and that of the IL22RA2 isoforms.
• Fig. S5. Analysis of the expression of ALDH1A2 in THP-1 cells and THP-1–derived DCs and of IL22RA2v2 expression in HL-60–derived neutrophils stimulated with Pam3CSK4 or LPS-EB.
• Table S1. Concentrations of the rhIL-22BP isoforms used to give 25-, 50-, and 100- fold molar excess compared to IL-22.
• Table S2. Sequences of the probes and primers used to quantify the expression of the IL22RA2 isoforms by qPCR analysis.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 514 KB

---

Citation: C. Lim, M. Hong, R. Savan, Human IL-22 binding protein isoforms act as a rheostat for IL-22 signaling. Sci. Signal. 9, ra95 (2016).

© 2016 American Association for the Advancement of Science