Supplementary Materials

Supplementary Materials for:

Superresolution imaging of the cytoplasmic phosphatase PTPN22 links integrin-mediated T cell adhesion with autoimmunity

Garth L. Burn, Georgina H. Cornish, Katarzyna Potrzebowska, Malin Samuelsson, Juliette Griffié, Sophie Minoughan, Mark Yates, George Ashdown, Nicolas Pernodet, Vicky L. Morrison, Cristina Sanchez-Blanco, Harriet Purvis, Fiona Clarke, Rebecca J. Brownlie, Timothy J. Vyse, Rose Zamoyska, Dylan M. Owen, Lena M. Svensson*, Andrew P. Cope*

*Corresponding author. Email: andrew.cope{at}kcl.ac.uk (A.P.C.); lena_m.svensson{at}med.lu.se (L.M.S.)

This PDF file includes:

  • Fig. S1. PTPN22 and its phosphorylated substrates localize to the leading edge of migrating T cells.
  • Fig. S2. TIRF microscopy confirms the colocalization of PTPN22 with its phosphorylated substrates.
  • Fig. S3. Engagement of integrin leads to the phosphorylation of PTPN22 substrates in migrating T cells.
  • Fig. S4. Csk declusters upon engagement of LFA-1 and associates with PTPN22 in migrating T cells.
  • Fig. S5. Analysis of cluster characteristics and the abundances of PTPN22-R620 and PTPN22-W620 in migrating T cells.
  • Fig. S6. PTPN22 associates with LFA-1 in an Lck-dependent manner.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.57 MB


Citation: G. L. Burn, G. H. Cornish, K. Potrzebowska, M. Samuelsson, J. Griffié, S. Minoughan, M. Yates, G. Ashdown, N. Pernodet, V. L. Morrison, C. Sanchez-Blanco, H. Purvis, F. Clarke, R. J. Brownlie, T. J. Vyse, R. Zamoyska, D. M. Owen, L. M. Svensson, A. P. Cope, Superresolution imaging of the cytoplasmic phosphatase PTPN22 links integrin-mediated T cell adhesion with autoimmunity. Sci. Signal. 9, ra99 (2016).

© 2016 American Association for the Advancement of Science