Supplementary Materials

Supplementary Materials for:

Lipoarabinomannan binding to lactosylceramide in lipid rafts is essential for the phagocytosis of mycobacteria by human neutrophils

Hitoshi Nakayama, Hidetake Kurihara, Yasu S. Morita, Taroh Kinoshita, Laura Mauri, Alessandro Prinetti, Sandro Sonnino, Noriko Yokoyama, Hideoki Ogawa, Kenji Takamori, Kazuhisa Iwabuchi*

*Corresponding author. Email: iwabuchi{at}juntendo.ac.jp

This PDF file includes:

  • Fig. S1. Phagocytosis of nonopsonized S. aureus by neutrophils.
  • Fig. S2. Analysis of the colocalization of LacCer and CD11b.
  • Fig. S3. Effects of mycobacterial lipid–coated beads on Lyn activation in neutrophils.
  • Fig. S4. Binding specificity of LM to LacCer.
  • Fig. S5. Subcellular localization of the granular marker proteins LAMP and LacCer.
  • Fig. S6. Determination of phagosome purity.
  • Fig. S7. Effects of mycobacterial lipid–coated beads on Hck activation in neutrophil phagosomes.
  • Fig. S8. Localization of LacCer and LAMP1 in mycobacteria-containing phagosomes.
  • Fig. S9. Electron microscopy analysis of LacCer, Hck, and phagosomes.
  • Fig. S10. Effect of Hck-specific siRNA on the phagocytosis of MAC.
  • Fig. S11. Localization of LacCer and Hck in mycobacteria-containing phagosomes.
  • Fig. S12. Effects of Hck-specific siRNA on the phagocytosis of LAM-coated beads.

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Citation: H. Nakayama, H. Kurihara, Y. S. Morita, T. Kinoshita, L. Mauri, A. Prinetti, S. Sonnino, N. Yokoyama, H. Ogawa, K. Takamori, K. Iwabuchi, Lipoarabinomannan binding to lactosylceramide in lipid rafts is essential for the phagocytosis of mycobacteria by human neutrophils. Sci. Signal. 9, ra101 (2016).

© 2016 American Association for the Advancement of Science