Supplementary Materials

Supplementary Materials for:

Impaired coordination between signaling pathways is revealed in human colorectal cancer using single-cell mass cytometry of archival tissue blocks

Alan J. Simmons, Cherié R. Scurrah, Eliot T. McKinley, Charles A. Herring, Jonathan M. Irish, M. Kay Washington, Robert J. Coffey, Ken S. Lau*

*Corresponding author. Email: ken.s.lau{at}vanderbilt.edu

This PDF file includes:

  • Fig. S1. Imaging of single-cell suspensions prepared by FFPE-DISSECT from embedded mouse intestinal tissue by bright-field and autofluorescence imaging.
  • Fig. S2. Whole-mount staining of embedded mouse intestinal tissue prepared by FFPE-DISSECT before single-cell disaggregation.
  • Fig. S3. Fluorescence cytometry of samples prepared by FFPE-DISSECT.
  • Fig. S4. Signals are preserved for up to 1 hour after excision.
  • Fig. S5. Fixation time does not affect signaling marker detection in FFPE.
  • Fig. S6. Villus signal comparison between FFPE-DISSECT, quantitative imaging, and quantitative immunoblotting.
  • Fig. S7. Replicates over multiple animals depicting relationships between apoptosis and signaling pathways using t-SNE and biplots.
  • Fig. S8. Cell type–specific signaling in the murine duodenal epithelium.
  • Fig. S9. RAS induction of MEK-ERK signaling induces goblet cell identity.
  • Fig. S10. Hematoxylin and eosin of MSS and MSI CRCs showing tissue areas occupied by tumors.
  • Fig. S11. Signaling in human normal colon and CRC.
  • Fig. S12. Differentiation in human normal colon and CRC.
  • Fig. S13. Evidence of fixative penetration.
  • Fig. S14. Analysis of the organization of signaling pathways in human specimens.
  • Table S1. Mouse antibody reagent panel.
  • Table S2. Summary of pathological characteristics of human colon cancer samples.
  • Table S3. Human antibody reagent panel.

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Citation: A. J. Simmons, C. R. Scurrah, E. T. McKinley, C. A. Herring, J. M. Irish, M. K. Washington, R. J. Coffey, K. S. Lau, Impaired coordination between signaling pathways is revealed in human colorectal cancer using single-cell mass cytometry of archival tissue blocks. Sci. Signal. 9, rs11 (2016).

© 2016 American Association for the Advancement of Science