Supplementary Materials for:

Loss of epithelial G_q and G₁₁ signaling inhibits TGFβ production but promotes IL-33–mediated macrophage polarization and emphysema

Alison E. John*, Michael R. Wilson, Anthony Habgood, Joanne Porte, Amanda L. Tatler, Anastasios Stavrou, Gino Miele, Lisa Jolly, Alan J. Knox, Masao Takata, Stefan Offermanns, R. Gisli Jenkin

*Corresponding author. Email: alison.john{at}nottingham.ac.uk

This PDF file includes:

• Fig. S1. Characterization of alveolar epithelial cell–specific, G protein–deficient mouse colonies.
• Fig. S2. Histological analysis of littermate control mice.
• Fig. S3. Loss of epithelial Gα_q/Gα₁₁ signaling leads to altered lung function.
• Fig. S4. Effect of the deficiency in epithelial G_q/₁₁ signaling on the concentrations of T_H2 cytokines and ST2 in the lung.
• Table S1. Sequences of genotyping primers.
• Table S2. Sequences of RT-PCR primers.

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Other Supplementary Material for this manuscript includes the following:

• Data File S1 (Microsoft Excel format). Affymetrix gene array data.

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Citation: A. E. John, M. R. Wilson, A. Habgood, J. Porte, A. L. Tatler, A. Stavrou, G. Miele, L. Jolly, A. J. Knox, M. Takata, S. Offermanns, R. G. Jenkins, Loss of epithelial G_q and G₁₁ signaling inhibits TGFβ production but promotes IL-33–mediated macrophage polarization and emphysema. Sci. Signal. 9, ra104 (2016).

© 2016 American Association for the Advancement of Science