Supplementary Materials

Supplementary Materials for:

Loss of epithelial Gq and G11 signaling inhibits TGFβ production but promotes IL-33–mediated macrophage polarization and emphysema

Alison E. John*, Michael R. Wilson, Anthony Habgood, Joanne Porte, Amanda L. Tatler, Anastasios Stavrou, Gino Miele, Lisa Jolly, Alan J. Knox, Masao Takata, Stefan Offermanns, R. Gisli Jenkin

*Corresponding author. Email: alison.john{at}nottingham.ac.uk

This PDF file includes:

  • Fig. S1. Characterization of alveolar epithelial cell–specific, G protein–deficient mouse colonies.
  • Fig. S2. Histological analysis of littermate control mice.
  • Fig. S3. Loss of epithelial Gαq/Gα11 signaling leads to altered lung function.
  • Fig. S4. Effect of the deficiency in epithelial Gq/11 signaling on the concentrations of TH2 cytokines and ST2 in the lung.
  • Table S1. Sequences of genotyping primers.
  • Table S2. Sequences of RT-PCR primers.

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Other Supplementary Material for this manuscript includes the following:

  • Data File S1 (Microsoft Excel format). Affymetrix gene array data.

Citation: A. E. John, M. R. Wilson, A. Habgood, J. Porte, A. L. Tatler, A. Stavrou, G. Miele, L. Jolly, A. J. Knox, M. Takata, S. Offermanns, R. G. Jenkins, Loss of epithelial Gq and G11 signaling inhibits TGFβ production but promotes IL-33–mediated macrophage polarization and emphysema. Sci. Signal. 9, ra104 (2016).

© 2016 American Association for the Advancement of Science