Supplementary Materials

Supplementary Materials for:

Stem-piped light activates phytochrome B to trigger light responses in Arabidopsis thaliana roots

Hyo-Jun Lee, Jun-Ho Ha, Sang-Gyu Kim, Han-Kyu Choi, Zee Hwan Kim, Yun-Jeong Han, Jeong-Il Kim, Youngjoo Oh, Variluska Fragoso, Kwangsoo Shin, Taeghwan Hyeon, Hong-Gu Choi, Kyung-Hwan Oh, Ian T. Baldwin,* Chung-Mo Park* *

*Corresponding author. Email: cmpark{at}snu.ac.kr (C.-M.P.); baldwin{at}ice.mpg.de (I.T.B.)

This PDF file includes:

  • Fig. S1. Schematic diagram of plant sample preparation for RNA sequencing.
  • Fig. S2. Expression of photoreceptor genes in roots.
  • Fig. S3. Plant growth for fluorescence imaging of phyB and HY5 distribution in root tissues.
  • Fig. S4. Lateral root formation in grafted Col-0 and hy5-221 plants.
  • Fig. S5. Primary root growth in soil-grown hy5-221 mutants.
  • Fig. S6. Effects of growth hormones and sucrose on the nuclear import of phyB and HY5 stability in root cells.
  • Fig. S7. Normalized light transmission through stem and stem-root segments.
  • Fig. S8. Transmission of lights with different spectral compositions through stem-root segments.
  • Fig. S9. Fluence rates and spectral compositions of stem-piped light.
  • Fig. S10. Plant growth under soil light block conditions.
  • Fig. S11. Experimental evaluation of the soil cover treatments.
  • Fig. S12. Spectral composition of FR-rich light.
  • Legends for tables S1 to S3
  • Table S4. Root genes that are differentially co-regulated by shoot light and root light.
  • Table S5. Primers used in this study.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Differentially expressed genes in the roots of plants having shoots in the dark and roots in the light.
  • Table S2 (Microsoft Excel format). Differentially expressed genes in the roots of plants having shoots in the light and roots in the dark.
  • Table S3 (Microsoft Excel format). Differentially expressed genes under both root-light and shoot-light conditions.

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Citation: H.-J. Lee, J.-H. Ha, S.-G. Kim, H.-K. Choi, Z. H. Kim, Y.-J. Han, J.-I. Kim, Y. Oh, V. Fragoso, K. Shin, T. Hyeon, H.-G. Choi, K.-H. Oh, I. T. Baldwin, C.-M. Park, Stem-piped light activates phytochrome B to trigger light responses in Arabidopsis thaliana roots. Sci. Signal. 9, ra106 (2016).

© 2016 American Association for the Advancement of Science