Supplementary Materials

Supplementary Materials for:

Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action

George D. Dickinson,* Kyle L. Ellefsen, Silvina Ponce Dawson, John E. Pearson, Ian Parker

*Corresponding author. Email: dickinsg{at}uci.edu

This PDF file includes:

  • Methods S1. Theoretical derivation of puff latencies
  • Methods S2. Calculation of range of action
  • Fig. S1. First-puff latencies depended strongly on the distance from the spot flash stimulation, whereas there was no significant distance dependence for distributed flash stimulation.
  • Fig. S2. Ca2+ waves evoked by photoreleased i-IP3 in SH-SY5Y cells without EGTA.
  • Fig. S3. Latencies of puffs evoked by spot photorelease of i-IP3 in COS-7 cells.
  • Fig. S4. Lengths of SH-SY5Y cells and spatial extent of the photolysis laser spot.
  • Fig. S5. Experimental and simulated first-puff latency data for the 100- and 200-ms spot flash durations.
  • Legend for movie S1
  • Reference (37)

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Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Puffs evoked by localized and distributed flash photorelease of IP3.

Citation: G. D. Dickinson, K. L. Ellefsen, S. P. Dawson, J. E. Pearson, I. Parker, Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action. Sci. Signal. 9, ra108 (2016).

© 2016 American Association for the Advancement of Science