Supplementary Materials

Supplementary Materials for:

RasGRP1 promotes amphetamine-induced motor behavior through a Rhes interaction network ("Rhesactome") in the striatum

Neelam Shahani, Supriya Swarnkar, Vincenzo Giovinazzo, Jenny Morgenweck, Laura M. Bohn, Catherina Scharager-Tapia, Bruce Pascal, Pablo Martinez-Acedo, Kshitij Khare, Srinivasa Subramaniam*

*Corresponding author. Email: ssubrama{at}scripps.edu

This PDF file includes:

  • Fig. S1. Identification of 49-kDa Rhes using LC-MS/MS.
  • Fig. S2. Rhes protein abundance in the striatum of wild-type and RasGRP1 mutant mice.
  • Fig. S3. Half-life of myc-tagged Rhes protein.
  • Fig. S4. Intracellular localization of Rhes and RasGRP1.
  • Fig. S5. Immunoprecipitation of Rhes by LC-MS/MS.
  • Fig. S6. Differential interaction of wild-type and mutant GST-Rhes with His-RasGRP1.
  • Fig. S7. Flow chart for IP–LC-MS/MS for the detection of a physiological Rhesactome.
  • Fig. S8. Validation of selected Rhes interactors.
  • Table S1. Group and number of samples analyzed by IP–LC-MS/MS.
  • Table S2. Comparison of known Rhes/RasGRP1 interactors to the striatal Rhesactome.
  • Legends for data files S1 to S4
  • References (97102)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Rhesactome of wild-type mice striatum in response to amphetamine.
  • Data file S2 (Microsoft Excel format). Rhesactome of Rhes+/−/Rasgrp1+/+ and Rhes+/−/Rasgrp1−/− mice striatum in response to amphetamine.
  • Data file S3 (Microsoft Excel format). Proteins identified in control IgG IP LC-MS/MS in the wild-type mice striatum (control 1).
  • Data file S4 (Microsoft Excel format). Proteins identified in Rhes IgG IP LC-MS/MS in the Rhes KO mice striatum (control 2).

[Download Data files S1 to S4]


Citation: N. Shahani, S. Swarnkar, V. Giovinazzo, J. Morgenweck, L. M. Bohn, C. Scharager-Tapia, B. Pascal, P. Martinez-Acedo, K. Khare, S. Subramaniam, RasGRP1 promotes amphetamine-induced motor behavior through a Rhes interaction network ("Rhesactome") in the striatum. Sci. Signal. 9, ra111 (2016).

© 2016 American Association for the Advancement of Science