Supplementary Matrials

Supplementary Materials for:

Selective control of up-regulated and down-regulated genes by temporal patterns and doses of insulin

Takanori Sano, Kentaro Kawata, Satoshi Ohno, Katsuyuki Yugi, Hiroaki Kakuda, Hiroyuki Kubota, Shinsuke Uda, Masashi Fujii, Katsuyuki Kunida, Daisuke Hoshino, Atsushi Hatano, Yuki Ito, Miharu Sato, Yutaka Suzuki, Shinya Kuroda*

*Corresponding author. Email: skuroda{at}bs.s.u-tokyo.ac.jp

This PDF file includes:

  • Fig. S1. Selection of the up-regulated IRGs and down-regulated IRGs.
  • Fig. S2. The expression amounts of the up-regulated IRGs and down-regulated IRGs measured by RNA-seq and qRT-PCR.
  • Fig. S3. Experimental design of insulin stimulation patterns based on in vivo temporal patterns of blood insulin.
  • Fig. S4. Hierarchical clustering of expression patterns of up-regulated IRGs and down-regulated IRGs.
  • Fig. S5. Responses of the IRGs to step, pulse, and ramp stimulations and the dose responses in experiments and in simulation.
  • Fig. S6. Decay time constant by pulse stimulation represents the time constant of a pathway.
  • Fig. S7. Sensitivity and time constants of the increased and decreased IRTs from the RNA-seq data.
  • Fig. S8. Histograms of CV of parameter values in the best five models.
  • Table S1. KEGG pathways in which five or more IRGs were mapped.
  • Table S2. Primer sequences used for qRT-PCR measurements.
  • Table S3. KEGG pathways in which the up-regulated and down-regulated IRGs were mapped.
  • Table S4. Parameters of the experimental IRG responses.
  • Table S5. Calculated parameters from experimental qRT-PCR data.
  • Table S6. Estimated model parameters and simulated EC50 at 120 or 240 min for the up-regulated and down-regulated IRGs.
  • Legend for Data files S1 to S8

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). RNA-seq data of the DETs, DRGs, IRTs, and IRGs.
  • Data file S2 (Microsoft Excel format). qRT-PCR data from biological triplicates used for parameter calculation and modeling.
  • Data file S3 (Microsoft Excel format). qRT-PCR data for the step stimulation that were used for hierarchical clustering.
  • Data file S4 (Microsoft Excel format). qRT-PCR data for the pulse stimulation that were for hierarchical clustering.
  • Data file S5 (Microsoft Excel format). qRT-PCR data for the ramp stimulation that were used for hierarchical clustering.
  • Data file S6 (Microsoft Excel format). RNA-seq data and parameters of the increased and decreased IRTs.
  • Data file S7 (Microsoft Excel format). Rat insulin clamp data.
  • Data file S8 (Microsoft Excel format). qRT-PCR data of rat liver for the insulin clamp experiments.

[Download Data files S1 to S8]


Citation: T. Sano, K. Kawata, S. Ohno, K. Yugi, H. Kakuda, H. Kubota, S. Uda, M. Fujii, K. Kunida, D. Hoshino, A. Hatano, Y. Ito, M. Sato, Y. Suzuki, S. Kuroda, Selective control of up-regulated and down-regulated genes by temporal patterns and doses of insulin. Sci. Signal. 9, ra112 (2016).

© 2016 American Association for the Advancement of Science