Supplementary Materials

Supplementary Materials for:

Dynamic pre-BCR homodimers fine-tune autonomous survival signals in B cell precursor acute lymphoblastic leukemia

M. Frank Erasmus, Ksenia Matlawska-Wasowska, Ichiko Kinjyo, Avanika Mahajan, Stuart S. Winter, Li Xu, Michael Horowitz, Diane S. Lidke, Bridget S. Wilson*

*Corresponding author. Email: bwilson{at}salud.unm.edu

This PDF file includes:

  • Fig. S1. Generation, purification, and characterization of anti-Igβ Fab′ probes.
  • Fig. S2. Viterbi plots of states observed for pairs of QD-bound receptors under autonomous signaling conditions.
  • Fig. S3. Comparison of MSD values for pre-BCRs tracked under different conditions.
  • Fig. S4. Comparison of off-rates for homotypic interactions between pre-BCRs in two different cell lines.
  • Fig. S5. 3D plot for pairs of QD probes showing correlated motion at wide separation distances.
  • Fig. S6. Galectin-1 purification, homology model, and diffusion profile with varying concentrations.
  • Fig. S7. Characterization of the binding of the anti-VpreB antibody to 697 cells.
  • Fig. S8. Apoptotic response to single-agent treatment with kinase inhibitors targeting the pre-BCR pathway.
  • Fig. S9. Apoptotic response to combination treatment with vincristine and inhibitors of the pre-BCR pathway.
  • Fig. S10. Apoptotic response to combination treatment with vincristine and a SHIP inhibitor.
  • Fig. S11. Quantitation of the amounts of VpreB and Igβ on the surface of different BCP-ALL cells.
  • Fig. S12. Sequence alignment reveals polymorphism among different cell lines and patient samples.
  • Table S1. Characteristics of BCP-ALL cells used in this study.
  • Table S2. SPT receptor diffusion statistics.
  • Table S3. SPT receptor dimer statistics.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Two-color SPT analysis of 697 cells depicting correlated motion and serial engagement.
  • Movie S2 (.mov format). Two-color SPT analysis of Nalm6 cells depicting correlated motion at wide (>200 nm) distances.
  • Movie S3 (.mov format). SPT analysis showing pre-BCR diffusion in 697 cells in the presence or absence of exogenous galectin-1.

Citation: M. F. Erasmus, K. Matlawska-Wasowska, I. Kinjyo, A. Mahajan, S. S. Winter, L. Xu, M. Horowitz, D. S. Lidke, B. S. Wilson, Dynamic pre-BCR homodimers fine-tune autonomous survival signals in B cell precursor acute lymphoblastic leukemia. Sci. Signal. 9, ra116 (2016).

© 2016 American Association for the Advancement of Science