Supplementary Materials

Supplementary Materials for:

Chemoattractant concentration–dependent tuning of ERK signaling dynamics in migrating neutrophils

Elizabeth R. Zhang, Shanshan Liu, Lani F. Wu, Steven J. Altschuler, Melanie H. Cobb*

*Corresponding author. Email: melanie.cobb{at}utsouthwestern.edu

This PDF file includes:

  • Fig. S1. Migration of cells across a linear gradient of fMLP.
  • Fig. S2. ERK activation remains increased in cells responding to 10 nM fMLP.
  • Fig. S3. The abundance of pp38 is reduced below basal amounts within 10 min of fMLP stimulation.
  • Fig. S4. ERK promotes, whereas p38 inhibits, the migration of neutrophils toward fMLP.
  • Fig. S5. Inhibition of p38 results in increased pERK abundance in neutrophils at late times after exposure to 10 or 100 nM fMLP.
  • Legends for movies S1 to S8

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). DMSO-treated human neutrophils migrating in a gradient of 0 to 500 nM fMLP.
  • Movie S2 (.avi format). PD0325901-treated human neutrophils migrating in a gradient of 0 to 500 nM fMLP.
  • Movie S3 (.avi format). BIRB-796–treated human neutrophils migrating in a gradient of 0 to 500 nM fMLP.
  • Movie S4 (.avi format). DMSO-treated control HL-60 cells migrating in a gradient of 0 to 500 nM fMLP.
  • Movie S5 (.avi format). PD0325901-treated HL-60 cells migrating in a gradient of 0 to 500 nM fMLP.
  • Movie S6 (.avi format). SCH772984-treated HL-60 cells migrating in a gradient of 0 to 500 nM fMLP.
  • Movie S7 (.avi format). SB203580-treated HL-60 cells migrating in a gradient of 0 to 500 nM fMLP.
  • Movie S8 (.avi format). BIRB-796–treated HL-60 cells migrating in a gradient of 0 to 500 nM fMLP.

Citation: E. R. Zhang, S. Liu, L. F. Wu, S. J. Altschuler, M. H. Cobb, Chemoattractant concentration–dependent tuning of ERK signaling dynamics in migrating neutrophils. Sci. Signal. 9, ra122 (2016).

© 2016 American Association for the Advancement of Science