Supplementary Materials

Supplementary Materials for:

Mitotic phosphotyrosine network analysis reveals that tyrosine phosphorylation regulates Polo-like kinase 1 (PLK1)

Danielle Caron, Dominic P. Byrne, Philippe Thebault, Denis Soulet, Christian R. Landry, Patrick A. Eyers, Sabine Elowe*

*Corresponding author. Email: sabine.elowe{at}crchudequebec.ulaval.ca

This PDF file includes:

  • Fig. S1. pTyr patterns are reproducible with multiple pTyr antibodies.
  • Fig. S2. Network analysis of mitotic pTyr sites.
  • Fig. S3. MYC-PLK1 expression and stable cell line generation.
  • Fig. S4. Characterization of T210D PLK1 (1–369) proteins (wild type, D194A, Y217F, and Y217E).
  • Fig. S5. Analysis of PLK1 phosphorylation by SRC in vitro.
  • Legends for tables S1 to S13
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Mitotic pTyr Network (PDF format)
  • Table S1 (Microsoft Excel format). Phosphoproteomic reference publications for collation of mitotic pTyr sites.
  • Table S2 (Microsoft Excel format). List of nonredundant pTyr sites identified from the publications in table S1.
  • Table S3 (Microsoft Excel format). CST mitotic data sets using pY enrichment approaches used for collation of mitotic pTyr sites.
  • Table S4 (Microsoft Excel format). List of nonredundant pTyr sites identified from the data sets in table S3.
  • Table S5 (Microsoft Excel format). CST mitotic data sets using general phosphoenrichment approach used for collation of mitotic pTyr sites.
  • Table S6 (Microsoft Excel format). List of nonredundant pTyr sites identified from the data sets in table S5.
  • Table S7 (Microsoft Excel format). Complete list of nonredundant mitotic pTyr sites collated in this study.
  • Table S8 (Microsoft Excel format). List of Tyr-phosphorylated spindle-associated proteins.
  • Table S9 (Microsoft Excel format). List of nonredundant pTyr sites identified on spindle proteins in this study.
  • Table S10 (Microsoft Excel format). Raw data from GO analysis of the individual clusters in Fig. 3.
  • Table S11 (Microsoft Excel format). Incidence of comodification of pTyr and other PTMs in Fig. 4.
  • Table S12 (Microsoft Excel format). List of Tyr-phosphorylated kinases identified among the mitotic and spindle data sets.
  • Table S13 (Microsoft Excel format). Alignment of the activation and P+1 loops of the human kinase complement showing conservation of the PLK1 Tyr217 residue.
  • Movie S1 (.mov format). Three-dimensional reconstruction of pTyr signals at the mitotic spindle.

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[Download Tables S1 to S13]

[Download Movie S1]


Citation: D. Caron, D. P. Byrne, P. Thebault, D. Soulet, C. R. Landry, P. A. Eyers, S. Elowe, Mitotic phosphotyrosine network analysis reveals that tyrosine phosphorylation regulates Polo-like kinase 1 (PLK1). Sci. Signal. 9, rs14 (2016).

© 2016 American Association for the Advancement of Science