Supplementary Materials

Supplementary Materials for:

Rac signal adaptation controls neutrophil mobilization from the bone marrow

Carlo Cosimo Campa, Giulia Germena, Elisa Ciraolo, Francesca Copperi, Anna Sapienza, Irene Franco, Alessandra Ghigo, Annalisa Camporeale, Augusta Di Savino, Miriam Martini, Alessia Perino, Remco T. A. Megens, Angela R. M. Kurz, Christoph Scheiermann, Markus Sperandio, Andrea Gamba,* Emilio Hirsch*

*Corresponding author. Email: emilio.hirsch{at}unito.it (E.H.); andrea.gamba{at}polito.it (A.G.)

This PDF file includes:

  • Fig. S1. Model prediction of neutrophil ET at different chemokine production and degradation rates.
  • Fig. S2. Analysis of CXCR4 and CXCR2 abundance.
  • Fig. S3. Ratio of the diameter of the central vein to the distance from the endosteum.
  • Fig. S4. Analysis of the abundance and activation of integrin subunits.
  • Fig. S5. Neutrophil and CD34+ cell analysis after EHT-1864 treatment.
  • Fig. S6. Cxcl12 and Cxcl2 mRNA expression after G-CSF injection.
  • Table S1. Parameter values used for in silico modeling of neutrophil mobilization.
  • Table S2. Rates of Rac1 and Rac2 activation induced by either CXCL12 or CXCL2 stimulation.
  • Table S3. Parameters extracted from the kinetics of drug-induced neutrophil mobilization.
  • References (8892)

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Citation: C. C. Campa, G. Germena, E. Ciraolo, F. Copperi, A. Sapienza, I. Franco, A. Ghigo, A. Camporeale, A. Di Savino, M. Martini, A. Perino, R. T. A. Megens, A. R. M. Kurz, C. Scheiermann, M. Sperandio, A. Gamba, E. Hirsch, Rac signal adaptation controls neutrophil mobilization from the bone marrow. Sci. Signal. 9, ra124 (2016).

© 2016 American Association for the Advancement of Science